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Human Adenylyl cyclase-associated protein 2 (CAP2) ELISA Kit

Human Adenylyl cyclase-associated protein 2 (CAP2) ELISA Kit

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Specification

Product name Human Adenylyl cyclase-associated protein 2 (CAP2) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Adenylyl cyclase-associated protein 2 (CAP2) ELISA Kit employs a two-site sandwich ELISA to quantitate CAP2 in samples. An antibody specific for CAP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCAP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CAP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CAP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative CAP2; 2810452G09Rik; adenylyl cyclase-associated protein 2

Product Properties

Kit components • Human Adenylyl cyclase-associated protein 2 microplate
• Human Adenylyl cyclase-associated protein 2 standard
• Human Adenylyl cyclase-associated protein 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Adenylyl cyclase-associated protein 2 (CAP2) ELISA Kit has high sensitivity and excellent specificity for detection of Human CAP2. No significant cross-reactivity or interference between Human CAP2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Adenylate Cyclase Associated Protein is an actin-binding protein that was originally identified as a binding partner for adenylate cyclase. It binds actin monomers and sequesters them from the polymerization process. The yeast ortholog of CAP is called Srv2. Adenylyl Cyclase Associated Protein 2 was identified by its similarity to the gene for human adenylyl cyclase-associated protein. The function of the protein encoded by this gene is unknown. However, the protein appears to be able to interact with adenylyl cyclase-associated protein and actin.
Gene ID 10486
Alternative CAP2; 2810452G09Rik; adenylyl cyclase-associated protein 2
Accession P40123

Image & description

Fig.1. Human Adenylyl cyclase-associated protein 2 (CAP2) Standard Curve.

Fig.1. Human Adenylyl cyclase-associated protein 2 (CAP2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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