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HSP27 Polyclonal Antibody

HSP27 Polyclonal Antibody

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Specification

Product name HSP27 Polyclonal Antibody
Immunogen Synthesized peptide derived from human HSP27 around the non-phosphorylation site of S15
Host Rabbit
Reactivity Human, Mouse, Rat
Applications IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative HSPB1; HSP27; HSP28; Heat shock protein beta-1; HspB1; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The protein encoded by HSPB1 (heat shock protein family B (small) member 1) is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in HSPB1 are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN).
Gene ID 3315
Alternative HSPB1; HSP27; HSP28; Heat shock protein beta-1; HspB1; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27
Others HSP27 Polyclonal Antibody detects endogenous levels of HSP27 protein.
Accession P04792
Observed Band(KD) 27

Image & description

Fig.1. Western blot analysis of 1) Hela, 2) C2C12,  3) Mouse Brain, 4) Rat Brain using HSP27 Polyclonal Antibody. Secondary antibody was diluted at 1:20000.

Fig.1. Western blot analysis of 1) Hela, 2) C2C12, 3) Mouse Brain, 4) Rat Brain using HSP27 Polyclonal Antibody. Secondary antibody was diluted at 1:20000.

Fig.2. Immunohistochemical analysis of paraffin-embedded Mouse Kidney Tissue using HSP27 Polyclonal Antibody.

Fig.2. Immunohistochemical analysis of paraffin-embedded Mouse Kidney Tissue using HSP27 Polyclonal Antibody.

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