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HNF-3α/β/γ (Acetyl Lys264/253/211) Polyclonal Antibody

HNF-3α/β/γ (Acetyl Lys264/253/211) Polyclonal Antibody

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Specification

Product name HNF-3α/β/γ (Acetyl Lys264/253/211) Polyclonal Antibody
Immunogen Synthesized peptide derived from the human HNF-3α/β/γ around the acetylation site of K264/253/211
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:20000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative FOXA1; HNF3A; TCF3A; Hepatocyte nuclear factor 3-alpha; HNF-3-alpha; HNF-3A; Forkhead box protein A1; Transcription factor 3A; TCF-3A; FOXA2; HNF3B; TCF3B; Hepatocyte nuclear factor 3-beta; HNF-3-beta; HNF-3B;Forkhead box protein A2; Transcription factor 3B; TCF-3B; FOXA3; HNF3G; TCF3G; Hepatocyte nuclear factor 3-gamma; HNF-3-gamma; HNF-3G; Fork head-related protein FKH H3; Forkhead box protein A3; Transcription factor 3G; TCF-3G

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background FOXA1 encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver.
Gene ID 3169
Alternative FOXA1; HNF3A; TCF3A; Hepatocyte nuclear factor 3-alpha; HNF-3-alpha; HNF-3A; Forkhead box protein A1; Transcription factor 3A; TCF-3A; FOXA2; HNF3B; TCF3B; Hepatocyte nuclear factor 3-beta; HNF-3-beta; HNF-3B;Forkhead box protein A2; Transcription factor 3B; TCF-3B; FOXA3; HNF3G; TCF3G; Hepatocyte nuclear factor 3-gamma; HNF-3-gamma; HNF-3G; Fork head-related protein FKH H3; Forkhead box protein A3; Transcription factor 3G; TCF-3G
Others Acetyl-HNF-3α/β/γ (K264/253/211) Polyclonal Antibody detects endogenous levels of HNF-3α/β/γ protein only when acetylated at K264/253/211.
Accession P55317
Observed Band(KD) 50

Image & description

Fig.1. Western Blot analysis of Jurkat cells using Acetyl-HNF-3α/β/γ (K264/253/211) Polyclonal Antibody. Secondary Antibody was diluted at 1:2000O.

Fig.1. Western Blot analysis of Jurkat cells using Acetyl-HNF-3α/β/γ (K264/253/211) Polyclonal Antibody. Secondary Antibody was diluted at 1:2000O.

Fig.2. Western Blot analysis of JK cells using Acetyl-HNF-3α/β/γ (K264/253/211) Polyclonal Antibody. Secondary Antibody was diluted at 1:20000.

Fig.2. Western Blot analysis of JK cells using Acetyl-HNF-3α/β/γ (K264/253/211) Polyclonal Antibody. Secondary Antibody was diluted at 1:20000.

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