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HLA-DOβ Polyclonal Antibody

HLA-DOβ Polyclonal Antibody

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Specification

Product name HLA-DOβ Polyclonal Antibody
Immunogen Synthesized peptide derived from the N-terminal region of human HLA-DOβ at AA range: 10-90
Host Rabbit
Reactivity Human
Applications ELISA, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:40000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative HLA-DOB; HLA class II histocompatibility antigen; DO beta chain; MHC class II antigen DOB

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background HLA-DOB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DOA) and a beta chain (DOB), both anchored in the membrane. It is located in intracellular vesicles. DO suppresses peptide loading of MHC class II molecules by inhibiting HLA-DM. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail.
Gene ID 3112
Alternative HLA-DOB; HLA class II histocompatibility antigen; DO beta chain; MHC class II antigen DOB
Others HLA-DOβ Polyclonal Antibody detects endogenous levels of HLA-DOβ protein.
Accession P13765
Observed Band(KD) 30

Image & description

Fig. Western Blot analysis of various cells using HLA-DOβ Polyclonal Antibody diluted at 1:1000.

Fig. Western Blot analysis of various cells using HLA-DOβ Polyclonal Antibody diluted at 1:1000.

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