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Histone H2A.Z Polyclonal Antibody

Histone H2A.Z Polyclonal Antibody

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Product name Histone H2A.Z Polyclonal Antibody
Immunogen Synthetic Peptide
Host Rabbit
Reactivity Mouse, Rat
Applications WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:1000-1:2000).
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen
Alternative H2AFZ; H2AZ; Histone H2A.Z; H2A/z

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. H2AFZ encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality.
Gene ID 3015
Alternative H2AFZ; H2AZ; Histone H2A.Z; H2A/z
Others The antibody detects endogenous Histone H2A.Z protein.
Accession P0C0S5
Observed Band(KD) 14

Image & description

Fig. Western blot analysis of 1) Rat Brain, 2) Mouse Brain tissue, diluted at 1:2000. Secondary antibody was diluted at 1:20000.

Fig. Western blot analysis of 1) Rat Brain, 2) Mouse Brain tissue, diluted at 1:2000. Secondary antibody was diluted at 1:20000.

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