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His-Tag Monoclonal Antibody

His-Tag Monoclonal Antibody

Views(106) Publications(0) Catalog no(ABM40141)
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Specification

Product name His-Tag Monoclonal Antibody
Immunogen Synthetic Peptide
Host Mouse
Applications WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:5000-1:10000).
Clonality Monoclonal
Preparation method The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Alternative His; 6 His epitope; Hexa His; HHHHHH epitope; Polyhistidine Tag

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The his-tag is a series of six to nine histidine residues generally fused to either the carboxy or amino terminus of a recombinant protein. The small size of the his-tag, compared with other common epitope tags, makes it less likely to obstruct the target protein’s structure or function and more suitable to use under denaturing conditions. The string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper. The binding to metal ions under specific buffer conditions, allows for the simple purification and detection of his-tagged proteins.
Alternative His; 6 His epitope; Hexa His; HHHHHH epitope; Polyhistidine Tag
Others The antibody detects C-terminal, internal, and N-terminal His-tag fusion proteins.

Image & description

Fig. Western blot analysis of His-Tag recombinant protein, diluted at 1:10000. 1) His-MBP recombinant protein, 2) His-Tag transfected 293T.

Fig. Western blot analysis of His-Tag recombinant protein, diluted at 1:10000. 1) His-MBP recombinant protein, 2) His-Tag transfected 293T.

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