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GSK3β (phospho Ser9) Polyclonal Antibody

GSK3β (phospho Ser9) Polyclonal Antibody

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Specification

Product name GSK3β (phospho Ser9) Polyclonal Antibody
Immunogen Synthesized peptide derived from human GSK3β around the phosphorylation site of S9
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative GSK3B; Glycogen synthase kinase-3 beta; GSK-3 beta; Serine/threonine-protein kinase GSK3B

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Glycogen synthase kinase 3 beta encoded by GSK3B is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of GSK3B may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for GSK3B.
Gene ID 2932
Alternative GSK3B; Glycogen synthase kinase-3 beta; GSK-3 beta; Serine/threonine-protein kinase GSK3B
Others Phospho-GSK3β (S9) Polyclonal Antibody detects endogenous levels of GSK3β protein only when phosphorylated at S9.
Accession P49841
Observed Band(KD) 47

Image & description

Fig.1. Western Blot analysis of various cells using Phospho-GSK3β (S9) Polyclonal Antibody diluted at 1:500.

Fig.1. Western Blot analysis of various cells using Phospho-GSK3β (S9) Polyclonal Antibody diluted at 1:500.

Fig.2. Western Blot analysis of RAT-kidney cells using Phospho-GSK3β (S9) Polyclonal Antibody diluted at 1:500.

Fig.2. Western Blot analysis of RAT-kidney cells using Phospho-GSK3β (S9) Polyclonal Antibody diluted at 1:500.

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