|Product name||FH Fumarase Monoclonal Antibody|
|Reactivity||Human, Mouse, Rat|
|Applications notes||Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:1000-1:2000), IF (1:100-1:200).|
|Preparation method||The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen|
|Molecular weight||50 KD|
|Storage buffer||PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.|
|Storage instructions||Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||Fumarate hydratase encoded by FH is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in FH can cause fumarase deficiency and lead to progressive encephalopathy.|
|Others||The antibody detects endogenous Flotillin-1 protein.|
Fig. Western blot analysis of 1) Mouse Brain Tissue, 2) Rat Brain tissue, diluted at 1:2000.
1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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