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FH Fumarase Monoclonal Antibody

FH Fumarase Monoclonal Antibody

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Product name FH Fumarase Monoclonal Antibody
Immunogen Recombinant Protein
Host Mouse
Reactivity Human, Mouse, Rat
Applications IF, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:1000-1:2000), IF (1:100-1:200).
Clonality Monoclonal
Preparation method The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 50 KD
Storage buffer PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Fumarate hydratase encoded by FH is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in FH can cause fumarase deficiency and lead to progressive encephalopathy.
Gene ID 2271
Others The antibody detects endogenous Flotillin-1 protein.
Accession P07954

Image & description

Fig. Western blot analysis of 1) Mouse Brain Tissue, 2) Rat Brain tissue, diluted at 1:2000.

Fig. Western blot analysis of 1) Mouse Brain Tissue, 2) Rat Brain tissue, diluted at 1:2000.

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