|Product name||ExKine™ Nuclei Extraction Kit (High Purity)|
|Applications notes||Abbkine ExKine™ Total Membrane Protein Extraction Kit is a simple, rapid and reproducible method to prepare cellular protein fractions highly enriched in membrane and hydrophobic proteins, based on temperature-dependent phase separation. The sample is first mixed and homogenized in the membrane protein extraction buffers. After a brief incubation at 37°C, the sample is centrifuged, producing an upper aqueous phase and a lower detergent-rich phase. Proteins that were anchored to the membrane or proteins containing one or two transmembrane domains are efficiently partitioned to the detergent-rich phase.|
|Kit components||• Extracion Buffer
• Wash Buffer
• Protease Inhibitor (100×)
|Features & Benefits||• Versatile—suitable for fresh mammalian cultured cells and tissues with little or no cross-contaminations.
• Fast and convenient—non-denatured, active proteins are purified in less than two hours.
• Compatible—apply in various downstream assays, including Western blotting, protein assays, enzyme activity assays, etc.
|Usage notes||Ensure all reagents and solutions are at the appropriate temperature before starting the assay.|
|Storage instructions||Refer to list of materials supplied for storage conditions of individual components. Stable for at least 12 months at recommended temperature from date of shipment. Gel pack with blue ice.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||The preparation of an extract from membrane is often the first step in studying membrane proteins and their interactions. The resulting preparation can be used directly in Western blotting, Dot blot, Immunoprecipitation, or as a starting point for the purification of specific proteins.|
Fig. Western blot of specific proteins fraction using Abbkine ExKine™ Total Membrane Protein Extraction Kit. Sample: 293T cells lysate. H: hydrophilic phase extraction was analyzed using Na+/K+-ATPase Antibody. M: Membrane protein extraction was analyzed using Na+/K+-ATPase Antibody.
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