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Elk-1 Polyclonal Antibody

Elk-1 Polyclonal Antibody

Views(53) Publications(0) Catalog no(ABP54098)
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Specification

Product name Elk-1 Polyclonal Antibody
Immunogen Synthesized peptide derived from human Elk-1 around the non-phosphorylation site of S389
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IHC-P, IP, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IP (2-5 ug/mg lysate), ELISA (1:20000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative ELK1; ETS domain-containing protein Elk-1

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background ELK1 is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum response element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. This gene produces multiple isoforms by using alternative translational start codons and by alternative splicing. Related pseudogenes have been identified on chromosomes 7 and 14.
Gene ID 2002
Alternative ELK1; ETS domain-containing protein Elk-1
Others Elk-1 Polyclonal Antibody detects endogenous levels of Elk-1 protein.
Accession P19419
Observed Band(KD) 45

Image & description

Fig. Western Blot analysis of KB cells using Elk-1 Polyclonal Antibody diluted at 1:500.

Fig. Western Blot analysis of KB cells using Elk-1 Polyclonal Antibody diluted at 1:500.

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