|Product name||EliKine™ Thyroxine (T4) ELISA Kit|
|Reactivity||Bovine, Chicken, Dog, Goat, Human, Monkey, Mouse, Rabbit, Rat, Sheep, Swine|
|Applications notes||EliKine™ T4 ELISA Kit employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to T4. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated T4. A competitive inhibition reaction is launched between T4 (Standards or samples) and Biotin-conjugated T4 with the pre-coated antibody specific for T4. The more amount of T4 in samples, the less antibody bound by Biotin-conjugated T4. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of T4 in the sample. The color development is stopped and the intensity of the color is measured.|
|SampleType||Other biological fluids, Plasma, Serum|
|Assay type||Competitive ELISA (quantitative)|
|Assay duration||Multiple steps standard competitive ELISA assay with a working time of 3-4 hours. It depends on the experience of the operation person.|
|Kit components||• T4 microplate
• T4 standard
• Biotin conjugated T4
• HRP substrate A
• HRP substrate B
• Stop solution
• Wash buffer
• Plate covers.
|Features & Benefits||EliKine™ T4 ELISA Kit has high sensitivity and excellent specificity for detection of T4. No significant cross-reactivity or interference between T4 and analogues was observed.|
|Calibration range||20 ng/ml-320 ng/ml|
|Limit of detection||10 ng/ml|
|Usage notes||• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for
|Storage instructions||The unopened kit should be stored at 2 - 8 °C. After opening, please store refer to protocols.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||T4 is a useful marker for the diagnosis of hypothyroidism and hyperthyroidism. The level of T4 is decreased in hypothyroid patients and is increased in hyperthyroid patients. The major form of thyroid hormone in the blood is thyroxine (T4), which has a longer half-life than T3. In humans, the ratio of T4 to T3 released into the blood is between 14:1 and 20:1. T4 is converted to the active T3 (three to four times more potent than T4) within cells by deiodinases (5'-iodinase). These are further processed by decarboxylation and deiodination to produce iodothyronamine (T1a) and thyronamine (T0a). All three isoforms of the deiodinases are selenium-containing enzymes, thus dietary selenium is essential for T3 production. Edward Calvin Kendall was responsible for the isolation of thyroxine in 1915.|
Fig.1. T4 Standard Curve.
Fig.2. EliKine™ T4 ELISA Kit is a competitive ELISA to quantitate T4 in samples.