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EliKine™ Rat TNF-α ELISA Kit

EliKine™ Rat TNF-α ELISA Kit

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Specification

Product name EliKine™ Rat TNF-α ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes EliKine™ Rat TNF-α ELISA Kit employs a two-site sandwich ELISA to quantitate TNF-α in samples. An antibody specific for TNF-α has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNF-α present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNF-α is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNF-α bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TNF-α; TNF; DADB-70P7.1; DIF; TNF-alpha; TNFA; TNFSF2; APC1 protein; TNF superfamily; member 2; TNF; macrophage-derived; TNF; monocyte-derived; cachectin; tumor necrosis factor alpha; TNFSF7

Product Properties

Kit components • Rat TNF-α microplate
• Rat TNF-α standard
• Rat TNF-α detect antibody
• EliKine™ Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers.
Features & Benefits EliKine™ Rat TNF-α ELISA Kit has high sensitivity and excellent specificity for detection of Rat TNF-α. No significant cross-reactivity or interference between Rat TNF-α and analogues was observed.
Calibration range 31.25 pg/mL- 2000 pg/mL
Limit of detection 16 pg/ml
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Tumor necrosis factor acts as a cytokine, binding TNF receptors and plays a role in regulation of cell proliferation, induction of apoptosis, and inflammatory response.
Gene ID 24835
Alternative TNF-α; TNF; DADB-70P7.1; DIF; TNF-alpha; TNFA; TNFSF2; APC1 protein; TNF superfamily; member 2; TNF; macrophage-derived; TNF; monocyte-derived; cachectin; tumor necrosis factor alpha; TNFSF7
Accession P16599

Image & description

Fig.1. Rat TNF-α Standard Curve.

Fig.1. Rat TNF-α Standard Curve.

Fig.2. EliKine™ Rat TNF-α ELISA Kit is a sandwich ELISA to quantitate TNFA in samples.

Fig.2. EliKine™ Rat TNF-α ELISA Kit is a sandwich ELISA to quantitate TNFA in samples.

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