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EliKine™ Rat Interferon Gamma (IFN-γ) ELISA Kit

EliKine™ Rat Interferon Gamma (IFN-γ) ELISA Kit

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Specification

Product name EliKine™ Rat Interferon Gamma (IFN-γ) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes EliKine™ Rat Interferon Gamma (IFN-γ) ELISA Kit employs a two-site sandwich ELISA to quantitate IFN-γ in samples. An antibody specific for IFN-γ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IFN-γ present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Rat IFN-γ detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of IFN-γ bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative IFG; IFI; IFN gamma; IFN Immune; IFNG; Interferon gamma; Interferon Gamma Precursor; Macrophage Activating Factor; MAF; T Cell Interferon; Type II Interferon

Product Properties

Kit components • Rat IFN-γ microplate
• Rat IFN-γ standard
• Rat IFN-γ detect antibody
• Standard diluent
• Sample diluent
• Chromogen solution A
• Chromogen solution B
• Stop solution
• Wash buffer
• Plate covers.
Features & Benefits EliKine™ Rat IFN-γ ELISA Kit has high sensitivity and excellent specificity for detection of Rat IFN-γ. No significant cross-reactivity or interference between Rat IFN-γ and analogues was observed.
Calibration range 150 pg/mL-2400 pg/mL
Limit of detection 15 pg/mL
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background IFN-gamma (Interferon-gamma) is the prototype proinflammatory cytokine and is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. IFN-gamma dimers signal through a receptor complex of two IFN-gamma R1 and two IFN-gamma R2 subunits.
Gene ID 25712
Alternative IFG; IFI; IFN gamma; IFN Immune; IFNG; Interferon gamma; Interferon Gamma Precursor; Macrophage Activating Factor; MAF; T Cell Interferon; Type II Interferon
Accession P01581

Image & description

Fig.1. Rat IFN-γ Standard Curve.

Fig.1. Rat IFN-γ Standard Curve.

Fig.2. EliKine™ Rat IFN-γ ELISA Kit is a sandwich ELISA to quantitate IFNG in samples.

Fig.2. EliKine™ Rat IFN-γ ELISA Kit is a sandwich ELISA to quantitate IFNG in samples.

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