| Product name | EliKine™ Rat IL-4 ELISA Kit |
| Reactivity | Rat |
| Applications | ELISA |
| Applications notes | EliKine™ Rat IL-4 ELISA Kit employs a two-site sandwich ELISA to quantitate IL-4 in samples. An antibody specific for IL-4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-4 is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-4 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Cell culture supernatants, Other biological fluids, Plasma, Serum |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
| Alternative | IL 4; IL4; BCGF-1; BCGF1; BSF1; IL-4; MGC79402; B cell growth factor 1; B_cell stimulatory factor 1; lymphocyte stimulatory factor 1 |
| Kit components | • Rat IL-4 microplate • Rat IL-4 standard • Rat IL-4 detect antibody • EliKine™ Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers. |
| Features & Benefits | EliKine™ Rat IL-4 ELISA Kit has high sensitivity and excellent specificity for detection of Rat IL-4. No significant cross-reactivity or interference between Rat IL-4 and analogues was observed. |
| Calibration range | 1.56 pg/mL-100 pg/mL |
| Limit of detection | 1.0 pg/mL |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate. |
| Storage instructions | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Interleukin 4 is a pleiotropic cytokine produced primarily by activated T lymphocytes,mast cells and basophils . IL-4 has multiple immune response-modulating functions on a variety of cell types. It is an important modulator of the differentiation of precursor T helper cells to the Th2 subset that mediates humoral immunity and modulates antibody production. In addition, IL-4 has also been shown to have anti-tumor activity both in vivoand in vitro.The biological effects of IL-4 are mediated by specific cell surface receptor complexes. One type of functional IL-4 receptor complex consists of the IL-4-binding subunit and a second chain, designated the common c chain because it has also been identified as a component of the receptor complexes for IL-4, IL-7, IL-9 and IL-15 |
| Gene ID | 287287 |
| Alternative | IL 4; IL4; BCGF-1; BCGF1; BSF1; IL-4; MGC79402; B cell growth factor 1; B_cell stimulatory factor 1; lymphocyte stimulatory factor 1 |
| Accession | P20096 |
Fig.1. Rat IL-4 Standard Curve.
Fig.2. EliKine™ Rat IL-4 ELISA Kit is a sandwich ELISA to quantitate IL4 in samples.
You must be logged in to post a review.
Reviews
There are no reviews yet.