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EliKine™ Rat IL-1β ELISA Kit

EliKine™ Rat IL-1β ELISA Kit

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Specification

Product name EliKine™ Rat IL-1β ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes EliKine™ Rat IL-1β ELISA Kit employs a two-site sandwich ELISA to quantitate IL-1β in samples. An antibody specific for IL-1β has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-1β present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-1β is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-1β bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative IL1B; IL-1; IL1-BETA; IL1F2; catabolin; preinterleukin 1 beta; pro-interleukin-1-beta

Product Properties

Kit components • Rat IL-1β microplate
• Rat IL-1β standard
• Rat IL-1β detect antibody
• EliKine™ Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers.
Features & Benefits EliKine™ Rat IL-1β ELISA Kit has high sensitivity and excellent specificity for detection of Rat IL-1β. No significant cross-reactivity or interference between Rat IL-1β and analogues was observed.
Calibration range 62.5 pg/ml-4000 pg/ml
Limit of detection 32 pg/mL
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Interleukin-1 (IL-1) is one of the first cytokines ever described. Its initial discovery was as a factor that could induce fever, control lymphocytes, increase the number of bone marrow cells and cause degeneration of bone joints. At this time, IL-1 was known under several other names including endogenous pyrogen, lymphocyte activating factor, haemopoetin-1 and mononuclear cell factor, amongst others. It was around 1984-1985 when scientists confirmed that IL-1 was actually composed of two distinct proteins, now called IL-1α and IL-1β.These belong to a family of cytokines known as the interleukin-1 superfamily.Both IL-1α and IL-1β are produced by macrophages, monocytes and dendritic cells.
Gene ID 24494
Alternative IL1B; IL-1; IL1-BETA; IL1F2; catabolin; preinterleukin 1 beta; pro-interleukin-1-beta
Accession Q5BKB0

Image & description

Fig.1. Rat IL-1β Standard Curve.

Fig.1. Rat IL-1β Standard Curve.

Fig.2. EliKine™ Rat IL-1β ELISA Kit is a sandwich ELISA to quantitate IL1B in samples.

Fig.2. EliKine™ Rat IL-1β ELISA Kit is a sandwich ELISA to quantitate IL1B in samples.

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