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EliKine™ Mouse IL-5 ELISA Kit

EliKine™ Mouse IL-5 ELISA Kit

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Specification

Product name EliKine™ Mouse IL-5 ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes EliKine™ Mouse IL-5 ELISA Kit employs a two-site sandwich ELISA to quantitate IL-5 in samples. An antibody specific for IL-5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-5 is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative IL 5; IL5; IL-5; IL-5T-cell replacing factor; interleukin-5; TRF; TRFB cell differentiation factor I

Product Properties

Kit components • Mouse IL-5 microplate
• Mouse IL-5 standard
• Mouse IL-5 detect antibody
• EliKine™ Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers.
Features & Benefits EliKine™ Mouse IL-5 ELISA Kit has high sensitivity and excellent specificity for detection of Mouse IL-5. No significant cross-reactivity or interference between Mouse IL-5 and analogues was observed.
Calibration range 7.8 pg/mL-500 pg/mL
Limit of detection 4 pg/mL
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background IL-5 (Interleukin-5) is a secreted cytokine that is expressed as a covalent dimer. It is produced by activated Th2 cells, eosinophils, mast cells, and iNKT cells and promotes eosinophilic and basophilic differentiation and activation. IL-5 signals through a receptor complex containing IL-5 R alpha and the Common beta Chain/CD131 on eosinophils and basophils. Naturally occurring soluble forms of IL-5 R alpha retain the ability to bind IL-5 and limit its bioavailability.
Gene ID 16191
Alternative IL 5; IL5; IL-5; IL-5T-cell replacing factor; interleukin-5; TRF; TRFB cell differentiation factor I
Accession P04401

Image & description

Fig.1. Mouse IL-5 Standard Curve.

Fig.1. Mouse IL-5 Standard Curve.

Fig.2. EliKine™ Mouse IL-5 ELISA Kit is a sandwich ELISA to quantitate IL5 in samples.

Fig.2. EliKine™ Mouse IL-5 ELISA Kit is a sandwich ELISA to quantitate IL5 in samples.

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