| Product name | EliKine™ Mouse IL-10 ELISA Kit |
| Reactivity | Mouse |
| Applications | ELISA |
| Applications notes | EliKine™ Mouse IL-10 ELISA Kit employs a two-site sandwich ELISA to quantitate IL-10 in samples. An antibody specific for IL-10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-10 is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Cell culture supernatants, Other biological fluids, Plasma, Serum |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
| Alternative | IL 10; IL10; CSIF; IL-10; IL10A; MGC126450; MGC126451; TGIF; cytokine synthesis inhibitory factor |
| Kit components | • Mouse IL-10 microplate • Mouse IL-10 standard • Mouse IL-10 detect antibody • EliKine™ Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers. |
| Features & Benefits | EliKine™ Mouse IL-10 ELISA Kit has high sensitivity and excellent specificity for detection of Mouse IL-10. No significant cross-reactivity or interference between Mouse IL-10 and analogues was observed. |
| Calibration range | 93.75 pg/mL- 6000 pg/mL |
| Limit of detection | 50 pg/mL |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate. |
| Storage instructions | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Interleukin-10 is a small protein known as a cytokine that functions as an important regulator of the immune system. Although IL-10 is known to have many different roles in the immune system, its two major activities include inhibition of cytokine production by macrophages and inhibition of the accessory functions of macrophages during T cell activation. The effects of these actions cause IL-10 to play mainly an anti-inflammatory role in the immune system. IL-10 was originally known as the cytokine synthesis inhibiting factor (CSIF), and the discovery of this protein was based on its biological activity. IL-10 is mainly produced by the Th2 subset of CD4+ helper cells. However, it is also produced by some activated B cells, some Th1 cells , activated macrophages, and some nonhematopoietic sources . |
| Gene ID | 16153 |
| Alternative | IL 10; IL10; CSIF; IL-10; IL10A; MGC126450; MGC126451; TGIF; cytokine synthesis inhibitory factor |
| Accession | P18893 |
Fig.1. Mouse IL-10 Standard Curve.
Fig.2. EliKine™ Mouse IL-10 ELISA Kit is a sandwich ELISA to quantitate IL10 in samples.
Author：L Zhang, R Dong, Y Wang, L Wang Publication name：Pharmaceutical Biology IF：6
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