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EliKine™ Human TIMP-1 ELISA Kit

EliKine™ Human TIMP-1 ELISA Kit

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Specification

Product name EliKine™ Human TIMP-1 ELISA Kit
Reactivity Human
Applications ELISA
Applications notes EliKine™ Human TIMP-1 ELISA Kit employs a two-site sandwich ELISA to quantitate TIMP-1 in samples. An antibody specific for TIMP-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TIMP-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TIMP-1 is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TIMP-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TIMP1; RP1-230G1.3; CLGI; EPA; EPO; FLJ90373; HCI; TIMP; erythroid potentiating activity;fibroblast collagenase inhibitor;Metalloproteinase inhibitor 1

Product Properties

Kit components • Human TIMP-1 microplate
• Human TIMP-1 standard
• Human TIMP-1 detect antibody
• EliKine™ Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers.
Features & Benefits EliKine™ Human TIMP-1 ELISA Kit has high sensitivity and excellent specificity for detection of Human TIMP-1. No significant cross-reactivity or interference between Human TIMP-1 and analogues was observed.
Calibration range 31.25 pg/ml-2000 pg/ml
Limit of detection 16 pg/mL
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background TIMP metallopeptidase inhibitor 1, a member of the TIMP family. The glycoprotein is a natural inhibitor of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function.Transcription of TIMP-1 is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that TIMP-1 inactivation is polymorphic in human females. TIMP-1 is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction.
Gene ID 7076
Alternative TIMP1; RP1-230G1.3; CLGI; EPA; EPO; FLJ90373; HCI; TIMP; erythroid potentiating activity;fibroblast collagenase inhibitor;Metalloproteinase inhibitor 1
Accession P01033

Image & description

Fig.1. Human TIMP-1 Standard Curve.

Fig.1. Human TIMP-1 Standard Curve.

Fig.2. EliKine™ Human TIMP-1 ELISA Kit is a sandwich ELISA to quantitate TIMP1 in samples.

Fig.2. EliKine™ Human TIMP-1 ELISA Kit is a sandwich ELISA to quantitate TIMP1 in samples.

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