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EliKine™ Human TGF-β1 ELISA Kit

EliKine™ Human TGF-β1 ELISA Kit

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Specification

Product name EliKine™ Human TGF-β1 ELISA Kit
Reactivity Human, Mouse, Rat
Applications ELISA
Applications notes EliKine™ Human TGF-β1 ELISA Kit employs a two-site sandwich ELISA to quantitate TGF-β1 in samples. An antibody specific for TGF-β1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TGF-β1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TGF-β1 is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TGF-β1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TGFB1; CED; DPD1; LAP; TGFB; TGFbeta; TGF-beta 1 protein; latency-associated peptide

Product Properties

Kit components • Human TGF-β1 microplate
• Human TGF-β1 standard
• Human TGF-β1 detect antibody
• EliKine™ Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers.
Features & Benefits EliKine™ Human TGF-β1 ELISA Kit has high sensitivity and excellent specificity for detection of Human TGF-β1. No significant cross-reactivity or interference between Human TGF-β1 and analogues was observed.
Calibration range 15.6 pg/ml-1000 pg/ml
Limit of detection 8 pg/mL
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background TGF-β is capable of producing a variety of effects and virtually all cell types respond to this factor in some way. The inappropriate presence of active TGF-β1 has been implicated in a variety of pathological conditions Because of the necessity for regulating its activity tightly, TGF-β is secreted by cells in the form of an inactive complex. This complex consists of TGF-β1 associated non-covalently with a protein designated the latency associated peptide (LAP). TGF-β1 and LAP represent components of a pro-peptide that is cleaved in a post-golgi compartment prior to secretion. LAP and TGF-β1 each consist of a disulfide-linked homodimer and the association of these two components renders TGF-β1 inactive and inaccessible to anti-TGF-β antibodies.
Gene ID 7040
Alternative TGFB1; CED; DPD1; LAP; TGFB; TGFbeta; TGF-beta 1 protein; latency-associated peptide
Accession P01137

Image & description

Fig.1. Human TGF-β1 Standard Curve.

Fig.1. Human TGF-β1 Standard Curve.

Fig.2. EliKine™ Human TGF-β1 ELISA Kit is a sandwich ELISA to quantitate TGFB1 in samples.

Fig.2. EliKine™ Human TGF-β1 ELISA Kit is a sandwich ELISA to quantitate TGFB1 in samples.

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