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EliKine™ Human Resistin ELISA Kit

EliKine™ Human Resistin ELISA Kit

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Specification

Product name EliKine™ Human Resistin ELISA Kit
Reactivity Human
Applications ELISA
Applications notes EliKine™ Human Resistin ELISA Kit employs a two-site sandwich ELISA to quantitate Resistin in samples. An antibody specific for Resistin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Resistin present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Resistin is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Resistin bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative HXCP 1; HXCP1; MGC126603; MGC126609; PRO1199; Resistin; Resistin delta2; RETN; RETN 1; RETN_HUMAN; RETN1; UNQ407; XCP 1; XCP1

Product Properties

Kit components • Human Resistin microplate
• Human Resistin standard
• Human Resistin detect antibody
• EliKine™ Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers.
Features & Benefits EliKine™ Human Resistin ELISA Kit has high sensitivity and excellent specificity for detection of Human Resistin. No significant cross-reactivity or interference between Human Resistin and analogues was observed.
Calibration range 31.25 pg/mL-2000 pg/mL
Limit of detection 16 pg/mL
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background RETN gene belongs to the family defined by the mouse resistin-like genes. The characteristic feature of this family is the C-terminal stretch of 10 cys residues with identical spacing. The mouse homolog of this protein is secreted by adipocytes, and may be the hormone potentially linking obesity to type II diabetes. Alternatively spliced transcript variants encoding the same protein have been found for RETN gene. 
Gene ID 56729
Alternative HXCP 1; HXCP1; MGC126603; MGC126609; PRO1199; Resistin; Resistin delta2; RETN; RETN 1; RETN_HUMAN; RETN1; UNQ407; XCP 1; XCP1
Accession Q9HD89

Image & description

Fig.1. Human Resistin Standard Curve.

Fig.1. Human Resistin Standard Curve.

Fig.2. EliKine™ Human Resistin ELISA Kit is a sandwich ELISA to quantitate RETN in samples.

Fig.2. EliKine™ Human Resistin ELISA Kit is a sandwich ELISA to quantitate RETN in samples.

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