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EliKine™ Human Prolactin ELISA Kit

EliKine™ Human Prolactin ELISA Kit

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Specification

Product name EliKine™ Human Prolactin ELISA Kit
Reactivity Human
Applications ELISA
Applications notes EliKine™ Human Prolactin ELISA Kit employs a two-site sandwich ELISA to quantitate Prolactin in samples. An antibody specific for prolactin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any prolactin present is bound by the immobilized antibody. After removing any unbound substances, a HRP conjugated antibody specific for prolactin is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of prolactin bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 2-3 hours. It depends on the experience of the operation person.
Alternative PRL; Prl1a1; Prol; Prolactin precursor; RATPRLSD1; RNPROL; RP23-351I6.2; RP3-404K8.1; OTTMUSP00000000651; Luteotropic Hormone; Lactogenic Hormone; Mammotropin; AV290867

Product Properties

Kit components • Human Prolactin microplate
• Human Prolactin standard
• Human Prolactin detect antibody
• HRP substrate A
• HRP substrate B
• Stop solution
• Wash buffer
• Plate covers.
Features & Benefits EliKine™ Human Prolactin ELISA Kit has high sensitivity and excellent specificity for detection of Human Prolactin. No significant cross-reactivity or interference between Human Prolactin and analogues was observed.
Calibration range 40 μIU/ml-2000 μIU/ml
Limit of detection 50 μIU/ml
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for
Storage instructions The unopened kit should be stored at 2 - 8 °C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Prolactin (gene name PRL) is a secreted neuroendocrine pituitary hormone that acts primarily on the mammary gland to promote lactation, but has pleiotropic effects in both males and females. Prolactin is predominantly found as 199 amino acid, 25 kDa glycosylated and 23 kDa non-glycosylated monomers. Human prolactin shares only 60% and 63% amino acid sequence identity with mouse and rat prolactin, respectively, although rat prolactin can activate the human prolactin recepto. Although better characterized in rodents than humans, post-translational modifcations such as polymerization, glycosylation, and proteolytic cleavage can alter the activities of prolactin. Non-glycosylated prolactin is produced by the pituitary gland and packaged in storage granules before secretion. Glycosylated prolactin is reported to be constitutively secreted, have lower biological potency, and be removed from the circulation more quickly.
Gene ID 5617
Alternative PRL; Prl1a1; Prol; Prolactin precursor; RATPRLSD1; RNPROL; RP23-351I6.2; RP3-404K8.1; OTTMUSP00000000651; Luteotropic Hormone; Lactogenic Hormone; Mammotropin; AV290867
Accession P01236

Image & description

Fig.1. Human Prolactin Standard Curve.

Fig.1. Human Prolactin Standard Curve.

Fig.2. EliKine™ Human Prolactin ELISA Kit is a sandwich ELISA to quantitate Prolactin in samples.

Fig.2. EliKine™ Human Prolactin ELISA Kit is a sandwich ELISA to quantitate Prolactin in samples.

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