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EliKine™ Human LH ELISA Kit

EliKine™ Human LH ELISA Kit

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Specification

Product name EliKine™ Human LH ELISA Kit
Reactivity Human
Applications ELISA
Applications notes EliKine™ Human LH ELISA Kit employs a two-site sandwich ELISA to quantitate LH in samples. An antibody specific for LH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LH present is bound by the immobilized antibody. After removing any unbound substances, a HRP conjugated antibody specific for LH is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 2-3 hours. It depends on the experience of the operation person.
Alternative LH-B; LHB; LSH-B; LSH-beta; LSHB_HUMAN; Luteinizing hormone subunit beta; Lutropin beta chain; Lutropin subunit beta

Product Properties

Kit components • Human LH microplate
• Human LH standard
• Human LH detect antibody
• HRP substrate A
• HRP substrate B
• Stop solution
• Wash buffer
• Plate covers.
Features & Benefits EliKine™ Human LH ELISA Kit has high sensitivity and excellent specificity for detection of Human LH. No significant cross-reactivity or interference between Human LH and analogues was observed.
Calibration range 2 IU/L-75 IU/L
Limit of detection 0.5 IU/L
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8 °C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Luteinizing hormone (LH) is produced in both men and women from the anterior pituitary gland in response to luteinizing hormonereleasing hormone (LH-RH or Gn-RH), that is released by the hypothalamus. LH, also called interstitial cell-stimulating hormone (ICSH) in men, is glycoprotein with a molecular weight of approximately 30,000 Dalton. LH stimulates ovulation and ovarian steroid production in the female. In the male, LH controls Leydig cell secretion of testosterone. LH is elevated in Luteal phase of menstrual cycle, primary hypogonadism, Gonadotropin-secreting pituitary tumors and menopause. LH is deceased in hypothalamic Gn-RH deficiency, pituitary LH deficiency and ectopic steroid production.
Gene ID 3972
Alternative LH-B; LHB; LSH-B; LSH-beta; LSHB_HUMAN; Luteinizing hormone subunit beta; Lutropin beta chain; Lutropin subunit beta
Accession P01229

Image & description

Fig.1. Human LH Standard Curve.

Fig.1. Human LH Standard Curve.

Fig.2. EliKine™ Human LH ELISA Kit is a sandwich ELISA to quantitate LH in samples.

Fig.2. EliKine™ Human LH ELISA Kit is a sandwich ELISA to quantitate LH in samples.

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