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EliKine™ Human Leptin ELISA Kit

EliKine™ Human Leptin ELISA Kit

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Specification

Product name EliKine™ Human Leptin ELISA Kit
Reactivity Human
Applications ELISA
Applications notes EliKine™ Human Leptin  ELISA Kit employs a two-site sandwich ELISA to quantitate Leptin  in samples. An antibody specific for Leptin  has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Leptin  present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Leptin  is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Leptin  bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative FLJ94114; LEP; LEP_HUMAN; LEPD; Leptin; Leptin Precursor Obesity Factor; OB; Obese protein

Product Properties

Kit components • Human Leptin  microplate
• Human Leptin  standard
• Human Leptin  detect antibody
• EliKine™ Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers.
Features & Benefits EliKine™ Human Leptin  ELISA Kit has high sensitivity and excellent specificity for detection of Human Leptin . No significant cross-reactivity or interference between Human Leptin  and analogues was observed.
Calibration range 31.25 pg/mL- 2000 pg/mL
Limit of detection 16 pg/mL
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background LEP gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in LEP gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. LEP gene has also been linked to type 2 diabetes mellitus development.
Gene ID 3952
Alternative FLJ94114; LEP; LEP_HUMAN; LEPD; Leptin; Leptin Precursor Obesity Factor; OB; Obese protein
Accession P41159

Image & description

Fig.1. Human Leptin Standard Curve.

Fig.1. Human Leptin Standard Curve.

Fig.2. EliKine™ Human Leptin ELISA Kit is a sandwich ELISA to quantitate LEP in samples.

Fig.2. EliKine™ Human Leptin ELISA Kit is a sandwich ELISA to quantitate LEP in samples.

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