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EliKine™ Human IFN-α ELISA Kit

EliKine™ Human IFN-α ELISA Kit

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Specification

Product name EliKine™ Human IFN-α ELISA Kit
Reactivity Human
Applications ELISA
Applications notes EliKine™ Human IFN-α ELISA Kit employs a two-site sandwich ELISA to quantitate IFN-α in samples. An antibody specific for IFN-α has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IFN-α present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IFN-α is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IFN-α bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative IFNA1; IFL; IFN; IFNA; IFNA13; IFN-ALPHA; IFN-alphaD

Product Properties

Kit components • Human IFN-α microplate
• Human IFN-α standard
• Human IFN-α detect antibody
• EliKine™ Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers.
Features & Benefits EliKine™ Human IFN-α ELISA Kit has high sensitivity and excellent specificity for detection of Human IFN-α. No significant cross-reactivity or interference between Human IFN-α and analogues was observed.
Calibration range 46.9 pg/mL - 3000 pg/mL
Limit of detection 46.9 pg/mL
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The protein encoded by IFN-α gene is produced by macrophages and has antiviral activity. IFN-α gene is intronless and the encoded protein is secreted.
Gene ID 3439
Alternative IFNA1; IFL; IFN; IFNA; IFNA13; IFN-ALPHA; IFN-alphaD
Accession P01562

Image & description

Fig.1. Human IFN-α Standard Curve.

Fig.1. Human IFN-α Standard Curve.

Fig.2. EliKine™ Human IFN-α ELISA Kit is a sandwich ELISA to quantitate IFN in samples.

Fig.2. EliKine™ Human IFN-α ELISA Kit is a sandwich ELISA to quantitate IFN in samples.

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