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EliKine™ Human HGF ELISA Kit

EliKine™ Human HGF ELISA Kit

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Specification

Product name EliKine™ Human HGF ELISA Kit
Reactivity Human
Applications ELISA
Applications notes EliKine™ Human HGF ELISA Kit employs a two-site sandwich ELISA to quantitate HGF in samples. An antibody specific for HGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HGF is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HGF bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative DFNB39; F TCF; Fibroblast derived tumor cytotoxic factor; Hepatocyte growth factor; Hepatopoietin A; Hepatopoietin-A; Hgf; HGFB; HPTA

Product Properties

Kit components • Human HGF microplate
• Human HGF standard
• Human HGF detect antibody
• EliKine™ Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers.
Features & Benefits EliKine™ Human HGF ELISA Kit has high sensitivity and excellent specificity for detection of Human HGF. No significant cross-reactivity or interference between Human HGF and analogues was observed.
Calibration range 125 pg/mL-8000 pg/mL
Limit of detection 60 pg/mL
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background HGF gene encodes a protein that binds to the hepatocyte growth factor receptor to regulate cell growth, cell motility and morphogenesis in numerous cell and tissue types. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate alpha and beta chains, which form the mature heterodimer. This protein is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. This protein also plays a role in angiogenesis, tumorogenesis, and tissue regeneration. Although the encoded protein is a member of the peptidase S1 family of serine proteases, it lacks peptidase activity. Mutations in HGF gene are associated with nonsyndromic hearing loss.
Gene ID 3082
Alternative DFNB39; F TCF; Fibroblast derived tumor cytotoxic factor; Hepatocyte growth factor; Hepatopoietin A; Hepatopoietin-A; Hgf; HGFB; HPTA
Accession P14210

Image & description

Fig.1. Human HGF Standard Curve.

Fig.1. Human HGF Standard Curve.

Fig.2. EliKine™ Human HGF ELISA Kit is a sandwich ELISA to quantitate HGF in samples.

Fig.2. EliKine™ Human HGF ELISA Kit is a sandwich ELISA to quantitate HGF in samples.

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