Login Register
English
0

Cart

$ 0

EliKine™ Human GM-CSF ELISA Kit

EliKine™ Human GM-CSF ELISA Kit

Views(223) Publications(0) Catalog no(KET6008)
Datasheet Print Share Email Share
  • Specification
  • FAQ
  • Publications(0)
  • Comments(0)

Specification

Product name EliKine™ Human GM-CSF ELISA Kit
Reactivity Human
Applications ELISA
Applications notes EliKine™ Human GM-CSF ELISA Kit employs a two-site sandwich ELISA to quantitate GM-CSF in samples. An antibody specific for GM-CSF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GM-CSF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GM-CSF is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GM-CSF bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative GM-CSF; GMCSF; Colony stimulating factor; Granulocyte Macrophage Colony Stimulating Factor; CSF 2; CSF2

Product Properties

Kit components • Human GM-CSF microplate
• Human GM-CSF standard
• Human GM-CSF detect antibody
• Avidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers.
Features & Benefits EliKine™ Human GM-CSF ELISA Kit has high sensitivity and excellent specificity for detection of Human GM-CSF. No significant cross-reactivity or interference between Human GM-CSF and analogues was observed.
Calibration range 11.72 pg/mL-750 pg/mL
Limit of detection 5 pg/mL
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. GM-CSF is used therapeutically in myeloid reconstitution following bone marrow transplant, bone marrow transplant engraftment failure or delay, mobilization and following transplantation of autologous peripheral blood progenitor cells, and following induction chemotherapy in older adults with acute myelogenous leukemia (AML).
Gene ID 1437
Alternative GM-CSF; GMCSF; Colony stimulating factor; Granulocyte Macrophage Colony Stimulating Factor; CSF 2; CSF2
Accession P04141

Image & description

Fig.1. Human GM-CSF Standard Curve.

Fig.1. Human GM-CSF Standard Curve.

Fig.2. EliKine™ Human GM-CSF ELISA Kit is a sandwich ELISA to quantitate GMCSF in samples.

Fig.2. EliKine™ Human GM-CSF ELISA Kit is a sandwich ELISA to quantitate GMCSF in samples.

This product has been cited in0publications

Reviews

There are no reviews yet.

Be the first to review “EliKine™ Human GM-CSF ELISA Kit”