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EliKine™ Human FSH ELISA Kit

EliKine™ Human FSH ELISA Kit

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Specification

Product name EliKine™ Human FSH ELISA Kit
Reactivity Human
Applications ELISA
Applications notes EliKine™ Human FSH ELISA Kit employs a two-site sandwich ELISA to quantitate FSH in samples. An antibody specific for FSH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FSH present is bound by the immobilized antibody. After removing any unbound substances, a HRP conjugated antibody specific for FSH is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FSH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 2-3 hours. It depends on the experience of the operation person.
Alternative Follicle stimulating hormone alpha chain; Follicle Stimulating Hormone Beta; Follicle stimulating hormone beta polypeptide; Follicle stimulating hormone beta subunit; Follitropin alpha chain; Follitropin beta chain; Follitropin subunit beta; FSH A; FSH alpha; FSH B; FSH beta; FSHA; FSHB

Product Properties

Kit components • Human FSH microplate
• Human FSH standard
• Human FSH detect antibody
• HRP substrate A
• HRP substrate B
• Stop solution
• Wash buffer
• Plate covers.
Features & Benefits EliKine™ Human FSH ELISA Kit has high sensitivity and excellent specificity for detection of Human FSH. No significant cross-reactivity or interference between Human FSH and analogues was observed.
Calibration range 2 IU/L-70 IU/L
Limit of detection 1.0 IU/L
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for
Storage instructions The unopened kit should be stored at 2 - 8 °C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Follicle Stimulating Hormone (FSH) is a glygoprotein produced by the anterior pituitary gland. In the female, FSH stimulates follicular growth, prepares ovarian follicles for action by LH and enhances the LH induced release of estrogen. FSH levels are elevated after menopause, castration and in premature ovarian failure. Although there are significant exceptions ovarian failure is indicated when random FSH concentrations exceed 40 mIU/ml. In the male, FSH stimulates seminiferous tubule and testicular growth and is involved in the early stages of spermatogenesis. Oligospermic males usually have elevated FSH levels. Tumors of the testes generally depress serum FSH concentrations, but levels of LH are elevated. High levels of FSH in men may be found in primary testicular failure and Klinefelter syndrome. Elevated concentrations are also present in cases of starvation, renal failure, hyperthyroidism, and cirrhosis.
Gene ID 2488
Alternative Follicle stimulating hormone alpha chain; Follicle Stimulating Hormone Beta; Follicle stimulating hormone beta polypeptide; Follicle stimulating hormone beta subunit; Follitropin alpha chain; Follitropin beta chain; Follitropin subunit beta; FSH A; FSH alpha; FSH B; FSH beta; FSHA; FSHB
Accession P01225

Image & description

Fig.1. Human FSH Standard Curve.

Fig.1. Human FSH Standard Curve.

Fig.2. EliKine™ Human FSH ELISA Kit is a sandwich ELISA to quantitate FSH in samples.

Fig.2. EliKine™ Human FSH ELISA Kit is a sandwich ELISA to quantitate FSH in samples.

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