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EliKine™ Human EGF ELISA Kit

EliKine™ Human EGF ELISA Kit

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Specification

Product name EliKine™ Human EGF ELISA Kit
Reactivity Human
Applications ELISA
Applications notes EliKine™ Human EGF ELISA Kit employs a two-site sandwich ELISA to quantitate EGF in samples. An antibody specific for EGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EGF is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EGF bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative Beta urogastrone; Epidermal growth factor; HOMG4; OTTHUMP00000219721; OTTHUMP00000219722; Pro epidermal growth factor; URG; Urogastrone

Product Properties

Kit components • Human EGF microplate
• Human EGF standard
• Human EGF detect antibody
• Avidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers.
Features & Benefits EliKine™ Human EGF ELISA Kit has high sensitivity and excellent specificity for detection of Human EGF. No significant cross-reactivity or interference between Human EGF and analogues was observed.
Calibration range 3.9 pg/mL-250 pg/mL
Limit of detection 2 pg/Ml
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Human EGF is a 53 amino acid long fragment of the extracellular domain of EGF precursor. As other group 1 members, EGF binds to, and activates the EGF receptor. This promotes EGFR dimerization. EGF has variety of diverse physiological effects. EGF activity is complicated by the fact that other EGF family members utilize EGF receptor. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. EGF is proposed to affect growth and/or differentiation of many other fetal and adult tissues.
Gene ID 1950
Alternative Beta urogastrone; Epidermal growth factor; HOMG4; OTTHUMP00000219721; OTTHUMP00000219722; Pro epidermal growth factor; URG; Urogastrone
Accession P01133

Image & description

Fig.1. Human EGF Standard Curve.

Fig.1. Human EGF Standard Curve.

Fig.2. EliKine™ Human EGF ELISA Kit is a sandwich ELISA to quantitate EGF in samples.

Fig.2. EliKine™ Human EGF ELISA Kit is a sandwich ELISA to quantitate EGF in samples.

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