EliKine™ Human CCL2 ELISA Kit

Product Information

  • Product name

    EliKine™ Human CCL2 ELISA Kit

  • Reactivity

    Human

  • Applications

    ELISA

  • Application notes

    EliKine™ Human CCL2 ELISA Kit employs a two-site sandwich ELISA to quantitate CCL2 in samples. An antibody specific for CCL2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CCL2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CCL2 is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CCL2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

  • Detection method

    Colorimetric

  • Sample type

    Cell culture supernatants, Other biological fluids, Plasma, Serum

  • Assay type

    Sandwich ELISA (quantitative)

  • Assay duration

    Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Fig.1. Human CCL2 Standard Curve.

Fig.2. EliKine™ Human CCL2 ELISA Kit is a sandwich ELISA to quantitate CCL2 in samples.

Product Properties

  • Kit components

    • Human CCL2 microplate
    • Human CCL2 standard
    • Human CCL2 detect antibody
    • Avidin-HRP
    • Standard diluent
    • Assay buffer
    • HRP substrate
    • Stop solution
    • Wash buffer
    • Plate covers.

  • Features & Benefits

    EliKine™ Human CCL2 ELISA Kit has high sensitivity and excellent specificity for detection of Human CCL2. No significant cross-reactivity or interference between Human CCL2 and analogues was observed.

  • Calibration range

    62.5 pg/ml-4000 pg/ml

  • Limit of detection

    30 pg/mL

  • Usage notes

    • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
    • Allow all reagents to warm to room temperature for at least 30 minutes before opening.
    • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
    • Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
    • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
    • It is recommended that all samples and standards be assayed in duplicate or triplicate.

  • Storage instructions

    The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

  • Shipping

    Gel pack with blue ice.

  • Precautions

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

  • Background

    CCL2 gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.

  • Gene ID

    6347

  • Alternative names

    CCL2; GDCF-2; HC11; HSMCR30; MCAF; MCP-1; MCP1; MGC9434; SCYA2; SMC-CF; monocyte chemoattractant protein-1; monocyte chemotactic and activating factor; monocyte secretory protein JE; small inducible cytokine

  • Accession

    P13500

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EliKine™ Human CCL2 ELISA Kit
Click to download booklet for more details

Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through support@abbkine.com if you need more information or support.

Cat #: KET6001

  • Size
  • Price
  • 48T $140
  • 96T $220
  • 96T*5 $1000

Contact information

Do not hesitate to contact us if you have any questions.

  •  Customer: service@abbkine.com
  •  Message: Click to Leave
  •  Tel: +86-27-59716789
  •  Fax: +86-27-59716788
  • Global Headquarters: Bldg 1, Harbour of Technology Times, No.35, Optical Valley Ave, Wuhan, Hubei, China. 430074

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