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EliKine™ Human β-hCG ELISA Kit

EliKine™ Human β-hCG ELISA Kit

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Specification

Product name EliKine™ Human β-hCG ELISA Kit
Reactivity Human
Applications ELISA
Applications notes EliKine™ Human β-hCG ELISA Kit employs a two-site sandwich ELISA to quantitate β-hCG in samples. An antibody specific for β-hCG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any β-hCG present is bound by the immobilized antibody. After removing any unbound substances, a HRP conjugated antibody specific for β-hCG is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of β-hCG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 2-3 hours. It depends on the experience of the operation person.
Alternative CGB; CGB3; choriogonadotropin subunit beta; CGB5; CGB7; CGB8; CG-Beta; Choriogonadotropin Subunit Beta; Chorionic gonadotrophin chain beta; chorionic gonadotropin beta 3 subunit; Chorionic Gonadotropin beta Chain; chorionic gonadotropin beta subunit; chorionic gonadotropin, beta polypeptide; hCGB

Product Properties

Kit components • Human β-hCG microplate
• Human β-hCG standard
• Human β-hCG detect antibody
• Urine Sample diluent
• HRP substrate A
• HRP substrate B
• Stop solution
• Wash buffer
• Plate covers.
Features & Benefits EliKine™ Human β-hCG ELISA Kit has high sensitivity and excellent specificity for detection of Human β-hCG. No significant cross-reactivity or interference between Human β-hCG and analogues was observed.
Calibration range 8 IU/L-240 IU/L
Limit of detection 2.0 IU/L
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8 °C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Human chorionic gonadotropin (hCG) is a glycoprotein hormone produced by trophoblastic cells of the placenta beginning 10 to 12 days after conception. Maintenance of the fetus in the first trimester of pregnancy requires the production of hCG, which binds to the corpus luteum of the ovary which is stimulated to produce progesterone which in turn maintains the secretory endometrium. hCG is present only in trace amounts in non pregnant urine and sera. It rises sharply during pregnancy. HCG is composed of two non identical, non covalently linked polypeptide chains designated as the a and b subunits. The a subunit of HCG is nearly identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH) and luteinizing hormone (LH). A germ cell tumor which is positive for cytokeratin, placental alkaline phosphatase (PLAP) and HCG but negative for EMA and AFP is probably a choriocarcinoma.
Gene ID 1082
Alternative CGB; CGB3; choriogonadotropin subunit beta; CGB5; CGB7; CGB8; CG-Beta; Choriogonadotropin Subunit Beta; Chorionic gonadotrophin chain beta; chorionic gonadotropin beta 3 subunit; Chorionic Gonadotropin beta Chain; chorionic gonadotropin beta subunit; chorionic gonadotropin, beta polypeptide; hCGB
Accession P0DN86

Image & description

Fig.1. Human β-hCG Standard Curve.

Fig.1. Human β-hCG Standard Curve.

Fig.2. EliKine™ Human β-hCG ELISA Kit is a sandwich ELISA to quantitate β-hCG in samples.

Fig.2. EliKine™ Human β-hCG ELISA Kit is a sandwich ELISA to quantitate β-hCG in samples.

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