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EGFR Monoclonal Antibody

EGFR Monoclonal Antibody

Views(42) Publications(0) Catalog no(ABM0013)
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Specification

Product name EGFR Monoclonal Antibody
Immunogen Synthetic Peptide
Host Mouse
Reactivity Human
Applications IF, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:1000), IF (1:100-1:200), IHC-P (1:50-1:200).
Clonality Monoclonal
Preparation method The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Alternative EGFR; ERBB; ERBB1; HER1; Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 170 KD
Storage buffer PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Epidermal growth factor receptor encoded by EGFR is a transmembrane glycoprotein that is a member of the protein kinase superfamily. Epidermal growth factor receptor is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of tepidermal growth factor receptor to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in EGFR are associated with lung cancer.
Gene ID 1956
Alternative EGFR; ERBB; ERBB1; HER1; Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1
Others The antibody detects endogenous EGFR proteins.
Accession P00533

Image & description

Fig.1. Western blot analysis of hela, diluted at 1:1000.

Fig.1. Western blot analysis of hela, diluted at 1:1000.

Fig.2. IF analysis of hela with antibody (Left) and DAPI (Right) diluted at 1:100.

Fig.2. IF analysis of hela with antibody (Left) and DAPI (Right) diluted at 1:100.

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