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Cytokeratin 18 Monoclonal Antibody

Cytokeratin 18 Monoclonal Antibody

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Specification

Product name Cytokeratin 18 Monoclonal Antibody
Immunogen Recombinant Protein
Host Mouse
Reactivity Human, Mouse, Rat
Applications IF, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:1000-1:3000), IF (1:100-1:200).
Clonality Monoclonal
Preparation method The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Alternative KRT18; CYK18; PIG46; Keratin, type I cytoskeletal 18; Cell proliferation-inducing gene 46 protein; Cytokeratin-18; CK-18; Keratin-18; K18

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 46 KD
Storage buffer PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in KRT18 have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for KRT18.
Gene ID 3875
Alternative KRT18; CYK18; PIG46; Keratin, type I cytoskeletal 18; Cell proliferation-inducing gene 46 protein; Cytokeratin-18; CK-18; Keratin-18; K18
Others The antibody detects endogenous Cytokeratin 18 protein.
Accession P05783

Image & description

Fig.1. Western blot analysis of 1) HepG2, 2) Hela, 3) Mouse Liver tissue, 4) C2C12, 5) Rat Heart tissue, 6) Mouse Skeletal Muscle tissue, diluted at 1:2000.

Fig.1. Western blot analysis of 1) HepG2, 2) Hela, 3) Mouse Liver tissue, 4) C2C12, 5) Rat Heart tissue, 6) Mouse Skeletal Muscle tissue, diluted at 1:2000.

Fig.2. IF analysis of Hela, and DAPI (Right) diluted at 1:100.

Fig.2. IF analysis of Hela, and DAPI (Right) diluted at 1:100.

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