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Collagen IV Mouse Monoclonal Antibody (5E10)

Collagen IV Mouse Monoclonal Antibody (5E10)

Views(62) Publications(0) Catalog no(ABM40372)
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Specification

Product name Collagen IV Mouse Monoclonal Antibody (5E10)
Immunogen Synthetic Peptide of Collagen IV
Host Mouse
Reactivity Human, Mouse, Rat
Applications IHC-P
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC-P (1:50-1:200).
Clonality Monoclonal
Preparation method The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Alternative Collagen alpha-1(IV) chain

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background COL4A1 (collagen type IV alpha 1 chain) encodes a type IV collagen alpha protein. Type IV collagen proteins are integral components of basement membranes. COL4A1 shares a bidirectional promoter with a paralogous gene on the opposite strand. The protein consists of an amino-terminal 7S domain, a triple-helix forming collagenous domain, and a carboxy-terminal non-collagenous domain. It functions as part of a heterotrimer and interacts with other extracellular matrix components such as perlecans, proteoglycans, and laminins. In addition, proteolytic cleavage of the non-collagenous carboxy-terminal domain results in a biologically active fragment known as arresten, which has anti-angiogenic and tumor suppressor properties. Mutations in COL4A1 cause porencephaly, cerebrovascular disease, and renal and muscular defects. Alternative splicing results in multiple transcript variants.
Gene ID 1282
Alternative Collagen alpha-1(IV) chain
Others The antibody detects endogenous Collagen IV protein.
Accession p02462
Observed Band(KD) 161

Image & description

Fig.1. Immunohistochemical analysis of paraffin-embedded Human Kidney Tissue using Collagen IV Mouse mAb diluted at 1:200.

Fig.1. Immunohistochemical analysis of paraffin-embedded Human Kidney Tissue using Collagen IV Mouse mAb diluted at 1:200.

Fig.2. Immunohistochemical analysis of paraffin-embedded Human Skin Tissue using Collagen IV Mouse mAb diluted at 1:200.

Fig.2. Immunohistochemical analysis of paraffin-embedded Human Skin Tissue using Collagen IV Mouse mAb diluted at 1:200.

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