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CK8 Monoclonal Antibody

CK8 Monoclonal Antibody

Views(44) Publications(0) Catalog no(ABM0019)
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Specification

Product name CK8 Monoclonal Antibody
Immunogen Synthetic Peptide
Host Mouse
Reactivity Human, Mouse, Rat
Applications IF, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:2000-1:5000), IF (1:50-1:200), IHC-P (1:200).
Clonality Monoclonal
Preparation method The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Alternative KRT8; CYK8; Keratin, type II cytoskeletal 8; Cytokeratin-8; CK-8; Keratin-8; K8; Type-II keratin Kb8

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 54 KD
Storage buffer PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background KRT8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of KRT8 typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. Keratin 8 plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in KRT8 cause cryptogenic cirrhosis. Alternative ly spliced transcript variants have been found for KRT8.
Gene ID 3856
Alternative KRT8; CYK8; Keratin, type II cytoskeletal 8; Cytokeratin-8; CK-8; Keratin-8; K8; Type-II keratin Kb8
Others The antibody detects endogenous CK8 proteins.
Accession P05787

Image & description

Fig.1. Western blot analysis of 1) Hela, 2) mouse brain, diluted at 1:4000.

Fig.1. Western blot analysis of 1) Hela, 2) mouse brain, diluted at 1:4000.

Fig.2. Immunohistochemical analysis of paraffin-embedded human colon tissue. 1, CK8 Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.2. Immunohistochemical analysis of paraffin-embedded human colon tissue. 1, CK8 Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.3. Immunohistochemical analysis of paraffin-embedded mouse liver tissue. 1, CK8 Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.3. Immunohistochemical analysis of paraffin-embedded mouse liver tissue. 1, CK8 Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.4. Immunohistochemical analysis of paraffin-embedded rat liver tissue. 1, CK8 Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.4. Immunohistochemical analysis of paraffin-embedded rat liver tissue. 1, CK8 Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

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