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CheKineTM Thioredoxin Peroxidase (TPX) Colorimetric Assay Kit

Product Information

  • Product name

    CheKineTM Thioredoxin Peroxidase (TPX) Colorimetric Assay Kit

  • Application notes

    TPX catalyzes H2O2 to oxidize dithiothreitol (DTT). The absorption wavelength of H2O2 is 240nm. TPX activity can be calculated by measuring the decrease rate of absorbance at 240nm and subtracting H2O2 catalyzed by catalase (CAT). Therefore, this kit can measure TPX and CAT activities of samples simultaneously.

Fig. CheKineTM Thioredoxin Peroxidase (TPX) Colorimetric Assay Kit

Product Properties

  • Kit components

    • Assay Buffer
    • Substrate
    • H2O2

  • Features & Benefits

    • Determination of TPX activity in serum, plasma, tissue/cell lysates and other biological fluids.

  • Usage notes

    • 96 Well Clear Flat Bottom UV-Transparent Microplate is needed.
    • The whole process needs to be carried out on ice, and the enzyme activity should be determined on the same day, to avoid repeated freeze-thaw of the homogenate solution.
    • In the detection of TPX activity in cells, the cell number must be between 3-5 x 106, and the extraction of TPX in cells can be followed by Assay Buffer grinding or ultrasonic treatment, cells cannot be treated by lysate.

  • Storage instructions

    Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 12 months from receipt. Refer to list of materials supplied for storage conditions of individual components.

  • Shipping

    Gel pack with blue ice.

  • Precautions

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

  • Background

    TPX belongs to the family of peroxidases. It mainly achieves anti-oxidation by reducing hydrogen peroxide and some hydroperoxides in the body. It functions similarly to GPX and is one of the key enzymes in the glutathione redox cycle. TPX is ubiquitous in various organisms, such as yeast, plants, animals, protozoa, parasites, bacteria, and archaea, and is highly conservative in evolution. TPX is closely related to the regulation of cell proliferation, differentiation, apoptosis and tumorigenesis. The main functions of TPX include cell detoxification, anti-oxidation, and regulation of signal transduction and immune response mediated by hydrogen peroxide.

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CheKineTM Thioredoxin Peroxidase (TPX) Colorimetric Assay Kit
Click to download booklet for more details

Notice:Protocol may vary from different lot which is subject to the manual along with the shipment. Please contact service@abbkine.com for your enquiry.

Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through support@abbkine.com if you need more information or support.

Cat #: KTB1660

  • Size
  • Price
  • 48T $80
  • 96T $120

Contact information

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