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CheKineTM Glutathione Oxidized (GSSG) Colorimetric Assay Kit

Product Information

  • Product name

    CheKineTM Glutathione Oxidized (GSSG) Colorimetric Assay Kit

  • Application notes

    Reduced glutathione can react with DTNB and generate 2-nitro-5-mercaptobenzoic acid, which has the maximum light absorption at 412nm wavelength. The original reduced glutathione in the sample is inhibited by 2-vinylpyridine, and then GSSG is reduced to GSH by Glutathione Reductase to determine the content of GSSG.

Fig. Standard Curve of GSSG in 96-well plate assay. The y-axis is ΔOD and the x-axis is GSSG concentration (µg/ml).

Product Properties

  • Kit components

    • Extraction Buffer
    • Inhibitor
    •Assay Buffer
    • GR
    • GR Cofactor
    • Chromogen
    • Standard

  • Features & Benefits

    • Determination of the content of GSSG in serum, plasma, tissue/cell lysates and other biological fluids.
    • A broad range linearity: 1-20 uM.
    • The minimum detection concentration is less than 1uM.

  • Usage notes

    • If the experiment is not carried out immediately, the sample can be kept at -80°C for 10 days.
    • All samples should make a 1:10 dilution with deionized water, and the result was multiplied by a factor of 10.
    • Always prepare fresh standards per use.
    • Diluted standard solution is unstable and must be used within 4 hours.

  • Storage instructions

    Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 12 months from receipt. Refer to list of materials supplied for storage conditions of individual components.

  • Shipping

    Gel pack with blue ice.

  • Precautions

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

  • Background

    Glutathione comes in two forms: reduced (GSH) and oxidized (GSSG). Glutathione oxidized is the oxidized form of Glutathione, also known as Dithioglutathione, which is formed by the oxidation of two reduced glutathione molecules. GSSG is reduced to GSH by Glutathione Reductase. Reduced glutathione exists in a large number of organisms and plays a major role. Measurement of GSH and GSSG content and GSH / GSSG ratio can reflect cells redox state, and it is also one main indicator of glutathione redox cycle.

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CheKineTM Glutathione Oxidized (GSSG) Colorimetric Assay Kit
Click to download booklet for more details

Notice:Protocol may vary from different lot which is subject to the manual along with the shipment. Please contact service@abbkine.com for your enquiry.

Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through support@abbkine.com if you need more information or support.

Cat #: KTB1610

  • Size
  • Price
  • 48T $80
  • 96T $136
  • 96T*5 $380

Contact information

Do not hesitate to contact us if you have any questions.

  •  Customer: service@abbkine.com
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  •  Fax: +86-27-59716788
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