- Product name
CheKine™ Uric Acid (UA) Colorimetric Assay Kit
- Application notes
The kit provides a simple method for detecting Uric Acid (UA) concentration in a variety of biological samples such as animal tissues, serum,urine and other biological fluids. In the assay, Uricase can catalyze UA to produce allantoin, CO2 and H2O2; H2O2 oxidizes Fe2+ in potassium ferrocyanide to produce Fe3+; Fe3+ further condenses with phenol and 4-aminoantipyrine to form red quinone compounds which has a characteristic absorption peak at 505 nm. The UA content can be calculated by measuring the light absorption at this wavelength.
- Kit components
• Extraction Buffer
• Reagent I A
• Reagent I B
- Features & Benefits
• Determination of Uric Acid (UA) concentration in a variety of biological samples such as animal tissues, serum,urine and other biological fluids.
• Detailed sample preparation and result calculation methods are provided.
• Simple operation steps.
- Usage notes
• Do not mix or substitute reagents or materials from other kit lots or vendors.
• Serum samples should be tested within 24 hours, or stored at 4°C and protected from light for no more than 72 hours.
• Ensure all reagents and equipment are at the appropriate temperature before starting the assay.
- Storage instructions
Storage at 4°C and protected from light upon receipt. Kit has a storage time of 6 months form receipt.
Gel pack with blue ice.
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Uric Acid (UA) is the main metabolite of birds and reptiles. The main product in normal human urine is urea with a small amount of uric acid. In addition, UA is also an important antioxidant, which can scavenge superoxide and hydroxyl radicals. The imbalance of UA production and excretion in the body can lead to the occurrence of many diseases. For example, an increase in blood UA can cause gout, renal damage and arteriosclerosis, while a decrease in UA can cause pernicious anemia, which is of great significance in clinical diagnosis.
- Alternative names
UA; Uric acid
Most popular with customers
CheKine™ Uric Acid (UA) Colorimetric Assay Kit
Click to download booklet for more details
Notice:Protocol may vary from different lot which is subject to the manual along with the shipment. Please contact email@example.com for your enquiry.
Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.
Preparation methods for Biochemical
Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.
Antibody application protocols
Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.
- ♦ Antibody Western Blotting (WB) protocol
- ♦ Antibody Immunohistochemistry (IHC) protocol
- ♦ Antibody Immunofluorescence (IF) protocol
- ♦ Antibody Immunoprecipitation (IP) protocol
- ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol
Protein&peptide usage suggestions
Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.
- ♦ Handling and storage suggestion for peptides and protein
- ♦ Cytokines and growth factors for cell culture application
Commonly used assay kits guidelines
Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.
We hope this will be helpful for your research work. Please let us know through firstname.lastname@example.org if you need more information or support.