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CheKine™ Toal Glutathione (T-GSH) Colorimetric Assay Kit

CheKine™ Toal Glutathione (T-GSH) Colorimetric Assay Kit

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Specification

Product name CheKine™ Toal Glutathione (T-GSH) Colorimetric Assay Kit
Applications notes GSSG is reduced to GSH using Glutathione Reductase (GR). GSH reacts with 5,5'-dithiobis-bis-(2-nitrobenoicacid) to form 2-nitrobenoicacid which has a characteristic absorption peak at 412 nm, the content of total glutathione is determined then. It can test samples of tissues, bacteria, fungus, cells, serums, ect.
Alternative Toal Glutathione; T-GSH; t-GSH

Product Properties

Kit components • Extraction Buffer
• Assay Buffer
• GR
• GR Cofactor
• Chromogen
• Standard
Features & Benefits • Determination of the content of total glutathione in a variety of biological samples such as tissues, bacteria, fungus, cells, serums, ect.
• Detailed sample preparation and result calculation methods are provided.
• Simple operation steps.
• Standard and standard curve line are provided.
Usage notes • Do not mix or substitute reagents or materials from other kit lots or different vendors, or it may cause abnormal result.
•Avoid foaming or bubbles when mixing or reconstituting components.
• To avoid cross-contamination, change pipette tips between additions of each standard level, between sample additions, and between reagent additions. Also, use separate reservoirs for each reagent.
•Ensure all reagents and solutions are at the appropriate temperature before starting the assay.
•Pls dilute the sample furtherly when the concentration of sample is higher than the maximal concentration of standard.
Storage instructions Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 12 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Glutathione is a tripeptide consisting of glutamate, cysteine and glycine. Glutathione contains sulfhydryl groups and has antioxidant and integrated detoxification effects. Glutathione has reduced form (GSH) or oxidized form (GSSG), reduced glutathione predominates in physiological conditions. Glutathione reductase catalyzes the alternations between the two forms. GSH/GSSG is one of the most important redox pairs in cells. Therefore, the determination of GSH and GSSG content and GSH/GSSG ratio in cells can well reflect the redox state of cells and is also one of the main indicators of the glutathione redox cycle.
Alternative Toal Glutathione; T-GSH; t-GSH

Image & description

Fig. CheKine™ Toal Glutathione (T-GSH) Colorimetric Assay Kit

Fig. CheKine™ Toal Glutathione (T-GSH) Colorimetric Assay Kit

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