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CheKine™ Tissue and Blood Alkaline Phosphatase (AKP/ALP) Colorimetric Assay Kit

CheKine™ Tissue and Blood Alkaline Phosphatase (AKP/ALP) Colorimetric Assay Kit

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Specification

Product name CheKine™ Tissue and Blood Alkaline Phosphatase (AKP/ALP) Colorimetric Assay Kit
Applications notes In an alkaline environment, AKP / ALP catalyzes phthalate disodium to generate free phenol; phenol reacts with 4-aminoantipyrine and potassium ferricyanide to produce a red quinone derivative, which has characteristic light absorption at 510 nm; the absorbance increase rate reflect AKP activity.

Product Properties

Kit components • Extraction Buffer
• Chromogen A
• Chromogen B
• Chromogen C
• Standard
Features & Benefits • Determination of alkaline phosphatase (AKP/ALP) activity in tissue lysates supernatants, plasma, serum, urine or purified enzyme samples.
• Sample preparation, experimental steps and result calculation are more detailed and accurate.
• Long shelf life.
Usage notes • Assay kit is intended for research use only. Not for use in diagnostic procedures. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.
• Do not mix or substitute kit reagents or materials from other lots or vendors. Kits are QC tested as a set of components and performance cannot be guaranteed if utilized separately or substituted.
• Avoid foaming or bubbles when mixing or reconstituting components.
• Avoid cross-contamination, change pipette tips between additions of standards, samples and reagents. Also, use separate reservoirs for each reagent.
• Ensure all reagents and equipment are at the appropriate temperature before assay.
• Chromogen A, Chromogen B and Chromogen C should be kept away from light. Chromogen C cannot be used after it turns blue-green.
• After adding Chromogen C, it must be mixed immediately, otherwise the color development will not be complete.
Storage instructions Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 12 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background AKP / ALP is a zinc-containing glycoprotease that can hydrolyze various natural and synthetic phospholipid monoester compounds in an alkaline environment. AKP / ALP is widely distributed in various organs of the human body, mainly the liver.

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