- Product name
CheKine™ Superoxide Dismutases (SOD) Assay Kit
- Application notes
CheKine™ Superoxide dismutase (SOD) Activity Assay Kit provides a simple and easy colorimetric assay for the quantitative determination of SOD enzyme activity in serum, plasma, tissue/cell lysates and other biological fluids. In the assay, superoxide anion (O2－) is provided by xanthine oxidase (XO) catalyzed reaction. O2－ reacts with a tetrazolium salt WST-8 dye to form a water-soluble colored formazan product. SOD scavenges the O2－ thus less O2－ is available for the chromogenic reaction. This inhibition activity of SOD is measured by colorimetric method at OD 450 nm.
- Kit components
• Assay Buffer
• Sample Diluent
• SOD Standard (200 U/mL)
• Xanthine Oxidase
- Features & Benefits
• Determination of SOD enzyme activity in serum, plasma, tissue/cell lysates and other biological fluids.
• Determining SOD activity by WST-8 method, the maximum inhibition percentage can be close to 100 %, and it can not be interfered by some common interfering factors.
• A broad range linearity: 200-3.13 U/ml.
- Usage notes
• Briefly centrifuge small vials at low speed prior to opening.
• Prior to assay, bring all reagents to room temperature (25°C). The Xanthine reagent may appear to be turbid. Briefly vortext this tube before pipetting.
• If not assayed immediately, samples can be stored at -80°C.
- Storage instructions
Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
Gel pack with blue ice.
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Superoxide dismutases (SOD, EC18.104.22.168) are enzymes that catalyze the dismutation of superoxide anion into O2 and H2O2. They are an important antioxidant defense against the toxicity of superoxide radical in all cells exposed to O2. Aberrant SOD activities have been linked to diseases such as amyotrophic lateral sclerosis, perinatal lethality, neural disorders and cancer. There are three major families of superoxide dismutase: Cu/Zn, Fe/Mn, and the Ni type. Three forms of superoxide dismutase are present in humans. SOD1 is located in the cytoplasm, SOD2 in the mitochondria, and SOD3 is extracellular.
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CheKine™ Superoxide Dismutases (SOD) Assay Kit
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Notice:Protocol may vary from different lot which is subject to the manual along with the shipment. Please contact firstname.lastname@example.org for your enquiry.
Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.
Preparation methods for Biochemical
Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.
Antibody application protocols
Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.
- ♦ Antibody Western Blotting (WB) protocol
- ♦ Antibody Immunohistochemistry (IHC) protocol
- ♦ Antibody Immunofluorescence (IF) protocol
- ♦ Antibody Immunoprecipitation (IP) protocol
- ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol
Protein&peptide usage suggestions
Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.
- ♦ Handling and storage suggestion for peptides and protein
- ♦ Cytokines and growth factors for cell culture application
Commonly used assay kits guidelines
Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.
We hope this will be helpful for your research work. Please let us know through email@example.com if you need more information or support.