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CheKine™ Superoxide Dismutases (SOD) Assay Kit

CheKine™ Superoxide Dismutases (SOD) Assay Kit

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Specification

Product name CheKine™ Superoxide Dismutases (SOD) Assay Kit
Applications notes Abbkine CheKine™ Superoxide dismutase (SOD) Activity Assay Kit provides a simple and easy colorimetric assay for the quantitative determination of SOD enzyme activity in serum, plasma, tissue/cell lysates and other biological fluids. In the assay, superoxide anion (O2-) is provided by xanthine oxidase (XO) catalyzed reaction. O2- reacts with a tetrazolium salt WST-8 dye to form a water-soluble colored formazan product. SOD scavenges the O2- thus less O2- is available for the chromogenic reaction. This inhibition activity of SOD is measured by colorimetric method at OD 450 nm.

Product Properties

Kit components • Assay Buffer
• Sample Diluent
• WST-8
• Enhancer
• SOD Standard (200 U/mL)
• Xanthine Oxidase
• Xanthine
Features & Benefits • Determination of SOD enzyme activity in serum, plasma, tissue/cell lysates and other biological fluids.
• Determining SOD activity by WST-8 method, the maximum inhibition percentage can be close to 100 %, and it can not be interfered by some common interfering factors.
• A broad range linearity: 3.13- 200U/mL.
Usage notes • Briefly centrifuge small vials at low speed prior to opening.
• Prior to assay, bring all reagents to room temperature (25°C). The Xanthine reagent may appear to be turbid. Briefly vortext this tube before pipetting.
• If not assayed immediately, samples can be stored at -80°C.
Storage instructions Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Superoxide dismutases (SOD, EC1.15.1.1) are enzymes that catalyze the dismutation of superoxide anion into O2 and H2O2. They are an important antioxidant defense against the toxicity of superoxide radical in all cells exposed to O2. Aberrant SOD activities have been linked to diseases such as amyotrophic lateral sclerosis, perinatal lethality, neural disorders and cancer. There are three major families of superoxide dismutase: Cu/Zn, Fe/Mn, and the Ni type. Three forms of superoxide dismutase are present in humans. SOD1 is located in the cytoplasm, SOD2 in the mitochondria, and SOD3 is extracellular.

Image & description

Fig. CheKine™ Superoxide Dismutases (SOD) Assay Kit

Fig. CheKine™ Superoxide Dismutases (SOD) Assay Kit

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