CheKine™ Superoxide anion Scavenging Capacity Assay Kit

Product Information

  • Product name

    CheKine™ Superoxide anion Scavenging Capacity Assay Kit

  • Application notes

    CheKine™ Superoxide anion Scavenging Assay Kit provides a simple and easy colorimetric assay for the study of Superoxide anion Scavenging ability in tissue/cell lysates and other biological fluids. In this assay, superoxide anion (O2-) is provided by xanthine oxidase (XO) catalyzed reaction. O2- reacts with a tetrazolium salt WST-8 dye to form a water-soluble colored formazan product, which can be easily quantified colorimetrically. Sample scavenges the O2- thus less O2- is available for the chromogenic reaction. This scavenging ability of sample is measured by colorimetric method at OD 450 nm.

Product Properties

  • Kit components

    • Assay Buffer
    • Sample Diluent
    • WST-8
    • Enhancer
    • Xanthine Oxidase
    • Xanthine

  • Features & Benefits

    • Determination the Superoxide anion Scavenging ability in various samples, including serum, plasma, tissue/cell lysates and other biological fluids.

  • Usage notes

    • Briefly centrifuge small vials at low speed prior to opening.
    • If not assayed immediately, samples can be stored at -80°C.

  • Storage instructions

    Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.

  • Shipping

    Gel pack with blue ice.

  • Precautions

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

  • Background

    The superoxide anion (O2-) is a short-lived radical production by the addition of an electron to oxygen. It is formed in response to environmental factors such as UV light, cigarette smoke, environmental pollutants, and g-radiation, or derived from oxidases like xanthine oxidase or NADPH oxidase. Once formed, O2- attacks cellular components and causes damage to lipids, proteins and DNA. This can initiate numerous diseases, including cancer, atherosclerosis, rheumatoid arthritis, diabetes, liver damage, and central nervous system disorders. The study of scavenging superoxide anion radicals has been gained more and more attention.

Most popular with customers

Reviews

There are no reviews yet.

Be the first to review “CheKine™ Superoxide anion Scavenging Capacity Assay Kit”

Your email address will not be published. Required fields are marked *

CheKine™ Superoxide anion Scavenging Capacity Assay Kit
Click to download booklet for more details

Notice:Protocol may vary from different lot which is subject to the manual along with the shipment. Please contact service@abbkine.com for your enquiry.

Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through support@abbkine.com if you need more information or support.

Cat #: KTB1080

  • Size
  • Price
  • 48T $80
  • 96T $120

Contact information

Do not hesitate to contact us if you have any questions.

  •  Customer: service@abbkine.com
  •  Message: Click to Leave
  •  Tel: +86-27-59716789
  •  Fax: +86-27-59716788
  • Global Headquarters: Bldg 1, Harbour of Technology Times, No.35, Optical Valley Ave, Wuhan, Hubei, China. 430074

Gaining Your Trust with our

High quality *
Attractive price
100% Guarantee
Secured shipment