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CheKine™ Superoxide anion Scavenging Capacity Assay Kit

CheKine™ Superoxide anion Scavenging Capacity Assay Kit

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Specification

Product name CheKine™ Superoxide anion Scavenging Capacity Assay Kit
Applications notes CheKine™ Superoxide anion Scavenging Assay Kit provides a simple and easy colorimetric assay for the study of Superoxide anion Scavenging ability in tissue/cell lysates and other biological fluids. In this assay, superoxide anion (O2-) is provided by xanthine oxidase (XO) catalyzed reaction. O2- reacts with a tetrazolium salt WST-8 dye to form a water-soluble colored formazan product, which can be easily quantified colorimetrically. Sample scavenges the O2- thus less O2- is available for the chromogenic reaction. This scavenging ability of sample is measured by colorimetric method at OD 450 nm.

Product Properties

Kit components • Assay Buffer
• Sample Diluent
• WST-8
• Enhancer
• Xanthine Oxidase
• Xanthine
Features & Benefits • Determination the Superoxide anion Scavenging ability in various samples, including serum, plasma, tissue/cell lysates and other biological fluids.
Usage notes • Briefly centrifuge small vials at low speed prior to opening.
• If not assayed immediately, samples can be stored at -80°C.
Storage instructions Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The superoxide anion (O2-) is a short-lived radical production by the addition of an electron to oxygen. It is formed in response to environmental factors such as UV light, cigarette smoke, environmental pollutants, and g-radiation, or derived from oxidases like xanthine oxidase or NADPH oxidase. Once formed, O2- attacks cellular components and causes damage to lipids, proteins and DNA. This can initiate numerous diseases, including cancer, atherosclerosis, rheumatoid arthritis, diabetes, liver damage, and central nervous system disorders. The study of scavenging superoxide anion radicals has been gained more and more attention.

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