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CheKine™ Superoxide Anion Colorimetric Assay Kit

Product Information

  • Product name

    CheKine™ Superoxide Anion Colorimetric Assay Kit

  • Application notes

    The kit provides a simple method for detecting the O2- content in a variety of biological samples such as animal tissues, plant tissues, serum, cells . In the assay, The superoxide anion in variety of biological samples reacts with hydroxylamine hydrochloride to produce NO2-, and NO2- reacts with Gris’ reagent. The mechanism of Gris’ analysis is summarized as the azo coupling between diazoniums, which is It is produced by sulfonamides and NO2- and N-(1-naphthyl)ethylenediamine dihydrochloride to generate a red azo compound with a characteristic absorption peak at 540nm. The O2- content in the sample can be calculated based on the A540 value.

Fig. Standard Curve of Superoxide Anio assay. The x-axis is OD540 and the y-axis is NO2-concentration (μM).

Product Properties

  • Kit components

    • Extraction Buffer • Reagent I • Reagent II • Reagent III • NaNO2 Standard (10mmoL/L)

  • Features & Benefits

    • Determination of the O2- content in a variety of biological samples such as animal tissues, plant tissues, serum, cells. • Detailed sample preparation and result calculation methods are provided. • Simple operation steps. • Standard and standard curve line are provided.

  • Calibration range

    0.0125-0.5 mg/mL

  • Limit of detection

    0.0125 mg/mL

  • Usage notes

    • Do not mix or substitute reagents or materials from other kit lots or vendors.
    • Avoid foaming or bubbles when mixing or reconstituting components.
    • Avoid cross-contamination, change pipette tips between additions of standards, samples and reagents. Also, use separate reservoirs for each reagent.
    • Ensure all reagents and equipment are at the appropriate temperature before starting the assay.
    • Please prepare chloroform by yourself.
    • Each sample needs to set up a control well to eliminate the influence of NO2-existing in the sample itself, so 96T can only measure 48 samples.

  • Storage instructions

    Storage at 4°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.

  • Shipping

    Gel pack with blue ice.

  • Precautions

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

  • Background

    Reactive oxygen species such as superoxide anions in organisms have immune and signal transduction effects, but when they accumulate too much, they will destroy cell membranes and biological macromolecules, leading to abnormal metabolism of cells and tissues in the body, causing many diseases.

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CheKine™ Superoxide Anion Colorimetric Assay Kit
Click to download booklet for more details

Notice:Protocol may vary from different lot which is subject to the manual along with the shipment. Please contact for your enquiry.

Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through if you need more information or support.

Cat #: KTB1210

  • Size
  • Price
  • 48T $69
  • 96T $119

Contact information

Do not hesitate to contact us if you have any questions.

  •  Customer:
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  •  Tel: +86-27-59716789
  •  Fax: +86-27-59716788
  • Global Headquarters: Bldg 1, Harbour of Technology Times, No.35, Optical Valley Ave, Wuhan, Hubei, China. 430074

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