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CheKine™ Superoxide Anion Colorimetric Assay Kit

CheKine™ Superoxide Anion Colorimetric Assay Kit

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Specification

Product name CheKine™ Superoxide Anion Colorimetric Assay Kit
Applications notes The kit provides a simple method for detecting the O2- content in a variety of biological samples such as animal tissues, plant tissues, serum, cells . In the assay, The superoxide anion in variety of biological samples reacts with hydroxylamine hydrochloride to produce NO2-, and NO2- reacts with Gris’ reagent. The mechanism of Gris’ analysis is summarized as the azo coupling between diazoniums, which is It is produced by sulfonamides and NO2- and N-(1-naphthyl)ethylenediamine dihydrochloride to generate a red azo compound with a characteristic absorption peak at 540nm. The O2- content in the sample can be calculated based on the A540 value.

Product Properties

Kit components • Extraction Buffer
• Reagent I
• Reagent II
• Reagent III
• NaNO2 Standard (10mmoL/L)
Features & Benefits • Determination of the O2- content in a variety of biological samples such as animal tissues, plant tissues, serum, cells.
• Detailed sample preparation and result calculation methods are provided.
• Simple operation steps.
• Standard and standard curve line are provided.
Calibration range 0.0125-0.5 mg/mL
Limit of detection 0.0125 mg/mL
Usage notes • Do not mix or substitute reagents or materials from other kit lots or vendors.
• Avoid foaming or bubbles when mixing or reconstituting components.
• Avoid cross-contamination, change pipette tips between additions of standards, samples and reagents. Also, use separate reservoirs for each reagent.
• Ensure all reagents and equipment are at the appropriate temperature before starting the assay.
• Please prepare chloroform by yourself.
• Each sample needs to set up a control well to eliminate the influence of NO2-existing in the sample itself, so 96T can only measure 48 samples.
Storage instructions Storage at 4°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Reactive oxygen species such as superoxide anions in organisms have immune and signal transduction effects, but when they accumulate too much, they will destroy cell membranes and biological macromolecules, leading to abnormal metabolism of cells and tissues in the body, causing many diseases.

Image & description

Fig. Standard Curve of Superoxide Anio assay. The x-axis is OD540 and the y-axis is NO2- concentration (μM).

Fig. Standard Curve of Superoxide Anio assay. The x-axis is OD540 and the y-axis is NO2- concentration (μM).

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