Product Information

• Product name

  CheKine™ Reduced Glutathione (GSH) Colorimetric Assay Kit

• Application notes

  DTNB reacts with reduced glutathione to form a yellow product. The optical density measured at 412 nm, can directly reflect glutathione concentration in the sample.

Fig.Standard Curve of GSH in 96-well plate assay. The y-axis is ΔOD and the x-axis is GSH concentration (µg/ml).

Product Properties

• Kit components

  • Extraction Buffer
  • Assay Buffer
  • Chromogen
  • Standard

• Features & Benefits

  • Determination of Reduced Glutathione concentration in serum, plasma, tissue/cell lysates and other biological fluids.
  • A broad range linearity: 2-200 µg/mL.
  • Measure concentration down to 2 µg/mL.

• Usage notes

  • The sample can be kept at -80°C for 10 days. If not do experiments immediately.
  • Always prepare fresh standards per use.
  • Diluted standard solution is unstable and must be used within 4 hours.

• Storage instructions

  Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 12 months from receipt. Refer to list of materials supplied for storage conditions of individual components.

• Shipping

  Gel pack with blue ice.

• Precautions

  The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

• Background

  Glutathione is a tripeptide of glycine, glutamic acid and cysteine. In the red blood cell, reduced glutathione (GSH) is the key to maintain hemoglobin in reduced state and protect cells from oxidative damage. GSH is the most important antioxidant sulfhydryl compound in cells, which plays an important role in oxidation resistance, protein sulfhydryl protection and amino acid transport across membrane. The ratio between reduced and oxidized glutathione (GSH / GSSG) is the main indicator of the cell's redox state. Therefore, measuring the content of GSH and GSSG and the ratio of GSH / GSSG in cells can reflect the redox state of cells.however, other enzymes with peroxidatic activity do not utilize these substrates.