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CheKine™ Pyruvate Acid (PA) Colorimetric Assay Kit

CheKine™ Pyruvate Acid (PA) Colorimetric Assay Kit

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Specification

Product name CheKine™ Pyruvate Acid (PA) Colorimetric Assay Kit
Applications notes The kit provides a simple method for detecting Pyruvate Acid (PA) concentration in a variety of biological samples such as animal tissues, plant tissues, cell culture: adherent or suspension cells, serum, bacterial. In the assay, Pyruvic acid reacts with 2,4-dinitrophenylhydrazine to generate pyruvate-2,4-dinitrophenylhydrazone, which appears brownish red under alkaline conditions and has a characteristic absorption peak at 520 nm. The Pyruvate Acid (PA) present in the sample is proportional to the signal obtained.
Alternative PA; Pyruvic acid

Product Properties

Kit components • Extraction Buffer
• Chromogen A
• Chromogen B
• Standard
Features & Benefits • Determination of Pyruvate Acid (PA) content in a variety of biological samples such as animal tissues, plant tissues, cell culture: adherent or suspension cells, serum, bacterial.
• Detailed sample preparation and result calculation methods are provided.
• Simple operation steps.
• Standard and standard curve line are provided.
Usage notes • Do not mix or substitute reagents or materials from other kit lots or vendors.
• The lowest detection limit is 1μg/mL or 1μg/g fresh weight or 10ng/mg prot.
• Ensure all reagents and equipment are at the appropriate temperature before starting the assay.
Storage instructions Storage at 4°C and protected from light upon receipt. Kit has a storage time of 6 months form receipt.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Pyruvate Acid (PA) is an important intermediate for the sugar metabolism in organisms and the mutual transformation of various substances in the body. It connects the three major metabolisms of glucose, fatty acids and amino acids through acetyl CoA and plays an important pivotal role.
Alternative PA; Pyruvic acid

Image & description

Fig. CheKine™ Pyruvate Acid (PA) Colorimetric Assay Kit

Fig. CheKine™ Pyruvate Acid (PA) Colorimetric Assay Kit

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