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CheKine™ Proline (PRO) Colorimetric Assay Kit

CheKine™ Proline (PRO) Colorimetric Assay Kit

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Specification

Product name CheKine™ Proline (PRO) Colorimetric Assay Kit
Applications notes The kit provides a simple method for detecting Pro content in a variety of biological samples such as serum, plasma, Plant or animal tissue, cells, and bacteria. In the assay, Pro is extracted with sulfosalicylic acid (SA). After heat treatment, Pro reacts with acidic ninhydrin solution to produce red; after extraction with toluene, the absorbance is measured at 520 nm.

Product Properties

Kit components • Extraction Buffer
• Assay Buffer I
•Chromogen
•Pro Substrate
Features & Benefits • Determination of Pro content in a variety of biological samples such as serum, plasma, Plant or animal tissue, cells, and bacteria.
• A broad range linearity: 0.5-80 μg/mL.
• Detailed sample preparation and result calculation methods are provided.
• Simple operation steps.
• Standard and standard curve line are provided.
Calibration range 0.5-80 μg/mL
Limit of detection 0.5 μg/mL
Usage notes • 96 Well Clear Flat Bottom UV-Transparent Microplate is needed.
• Do not mix or substitute reagents or materials from other kit lots or vendors.
• Avoid foaming or bubbles when mixing or reconstituting components.
• Avoid cross-contamination, change pipette tips between additions of standards, samples and reagents. Also, use separate reservoirs for each reagent.
• Ensure all reagents and equipment are at the appropriate temperature before starting the assay.
Storage instructions Storage at 4°C and protected from light upon receipt. Kit has a storage time of 12 months form receipt.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Pro is widely present in animals, plants, microorganisms and cultured cells. Under adversity conditions, the Pro content in plants increased significantly. The increased amount of Pro reflects stress resistance to a certain extent, and varieties with strong drought resistance tend to accumulate more proline. Therefore, the increased amount of proline can be used as one of the physiological indicators of stress resistance breeding.

Image & description

Fig. Standard Curve of Pro assay. The x-axis is OD520 and the y-axis is Pro concentration ( µg/mL).

Fig. Standard Curve of Pro assay. The x-axis is OD520 and the y-axis is Pro concentration ( µg/mL).

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