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CheKine™ Polyphenol Oxidase (PPO) Activity Colorimetric Assay Kit

CheKine™ Polyphenol Oxidase (PPO) Activity Colorimetric Assay Kit

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Specification

Product name CheKine™ Polyphenol Oxidase (PPO) Activity Colorimetric Assay Kit
Applications notes The kit provides a simple method for detecting PPO activity in a variety of biological samples such as serum, plasma, tissues, cells, plants and bacteria. In the assay, Polyphenol Oxidase (PPO) catalyzes catechol to generate quinone which has a characteristic absorption peak at 525 nm. The rate of quinone increase at 525 nm can reflect PPO activity.
Alternative PPO; Polyphenol Oxidase

Product Properties

Kit components • Extraction Buffer
• Reagent I
• Reagent II
• Reagent III
Features & Benefits • Determination of PPO activity in a variety of biological samples such as serum, plasma, tissues, cells, plants and bacteria.
• Detailed sample preparation and result calculation methods are provided.
• Simple operation steps.
Usage notes • Do not mix or substitute reagents or materials from other kit lots or vendors.
• The sample preparation must be done on ice to prevent the enzyme inactivation, and the enzyme activity should be determined on the same day. avoid repeated freeze-thaw.
• Ensure all reagents and equipment are at the appropriate temperature before starting the assay.
• The optimal reaction temperature of polyphenol oxidase for different samples is slightly different, and it can be adjusted between 25-37℃.
Storage instructions Storage at 4°C and protected from light upon receipt. Kit has a storage time of 12 months form receipt.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Polyphenol Oxidase (PPO) is widely found in animals, plants, microorganisms and cultured cells. As a copper-containing oxidase, PPO can causes browning by oxidizing monohydric phenols and dihydric phenols to produce quinones. This enzyme is also closely related to fruit and vegetable processing, tea quality and tissue culture.
Alternative PPO; Polyphenol Oxidase

Image & description

Fig. CheKine™ Polyphenol Oxidase (PPO) Activity 
Colorimetric Assay Kit

Fig. CheKine™ Polyphenol Oxidase (PPO) Activity Colorimetric Assay Kit

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