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CheKine™ Phenylalanine Ammonia Lyase (PAL) Activity Colorimetric Assay Kit

CheKine™ Phenylalanine Ammonia Lyase (PAL) Activity Colorimetric Assay Kit

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Specification

Product name CheKine™ Phenylalanine Ammonia Lyase (PAL) Activity Colorimetric Assay Kit
Applications notes The kit provides a simple method for detecting PAL activity in biological samples . In the assay, PAL catalyzes the cleavage of L-phenylalanine into trans-cinnamic acid and ammonia. Trans-cinnamic acid has a maximum absorption value at 290 nm. PAL activity is calculated by measuring the rate of increase in absorbance.

Product Properties

Kit components • Extraction Buffer
• Assay Buffer
• Substrate
• HCl
Features & Benefits • Determination of PAL activity in biological samples.
• Detailed sample preparation and result calculation methods are provided.
• Simple operation steps.
Usage notes • Do not mix or substitute reagents or materials from other kit lots or vendors.
• Avoid foaming or bubbles when mixing or reconstituting components.
• Avoid cross-contamination, change pipette tips between additions of standards, samples and reagents. Also, use separate reservoirs for each reagent.
• Ensure all reagents and equipment are at the appropriate temperature before starting the assay.
Storage instructions Storage at 4°C and protected from light upon receipt. Kit has a storage time of 12 months form receipt.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background PAL (EC4. 3 15) is widely present in various plants and a few microorganisms. It is a key enzyme and rate-limiting enzyme of phenylpropane metabolism in plants, and has not been found in animals. PAL is closely related to the synthesis of some important secondary substances such as lignin, isoflavone phytoales, and flavonoid pigments, and plays an important role in the normal growth and development of plants, disease resistance, and stress resistance.

Image & description

Fig. CheKine™ Phenylalanine Ammonia Lyase (PAL) Activity Colorimetric Assay Kit

Fig. CheKine™ Phenylalanine Ammonia Lyase (PAL) Activity Colorimetric Assay Kit

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