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CheKine™ NAD+ kinase (NADK) Activity Colorimetric Assay Kit

CheKine™ NAD+ kinase (NADK) Activity Colorimetric Assay Kit

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Specification

Product name CheKine™ NAD+ kinase (NADK) Activity Colorimetric Assay Kit
Applications notes The kit provides a simple method for detecting NADK activity in a variety of biological samples such as serum, plasma, tissues, cells, plants and bacteria. In the assay, NADK catalyzes the phosphorylation of NAD+ to produce NADP+; NADP+ can be reduced to NADPH by glucose-6-phosphate dehydrogenase. NADPH has a characteristic absorption peak at 340 nm. The rate of NADPH increase at 340 nm can reflect NADK activity.

Product Properties

Kit components • Extraction Buffer
• Assay Buffer I
• Assay Buffer II
• Substrate Mix
• Enzyme Mix
Features & Benefits • Determination of NADK activity in a variety of biological samples such as serum, plasma, tissues, cells, plants and bacteria.
• Detailed sample preparation and result calculation methods are provided.
• Simple operation steps.
Usage notes • 96 Well Clear Flat Bottom UV-Transparent Microplate is needed.
• Do not mix or substitute reagents or materials from other kit lots or vendors.
• The sample preparation must be done on ice to prevent the enzyme inactivation, and the enzyme activity should be determined on the same day. avoid repeated freeze-thaw.
• Substrate Mix and Enzyme Mix must be placed on ice during the measurement process. The reaction temperature has an influence on the results, so please keep it at 25°C (general species) or 37°C (mammal).
Storage instructions Storage at -20°C and protected from light upon receipt. Kit has a storage time of 12 months form receipt.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background NADK (EC 2.7.1.23) is widely found in animals, plants, microorganisms and cultured cells. It is the only enzyme that can catalyze the phosphorylation of NAD+ to NADP+ in organisms. It can catalyze the phosphorylation reaction of NAD(H) with ATP or inorganic polyphosphate [poly(P)] as a phosphoryl donor to generate NADP(H). Therefore, NAD kinase plays an important role in the synthesis of NADP(H) and the regulation of the balance between NAD(H) and NADP(H).

Image & description

Fig. CheKine™ NAD+ kinase (NADK) Activity Colorimetric Assay Kit

Fig. CheKine™ NAD+ kinase (NADK) Activity Colorimetric Assay Kit

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