|Product name||CheKine™ Monoamine Oxidase (MAO) Activity Colorimetric Assay Kit|
|Applications notes||The kit provides a simple method for detecting MAO activity in a variety of biological samples such as serum, plasma, tissues, cells, bacteria, plants and Fungi. In the assay, MAO catalyzes the deamination of monoamine substrates to the corresponding aldehydes, which are further oxidized to acid. The substrate has a characteristic absorption peak at 360 nm. The rate of monoamine substrates decrease at 360 nm can reflect MAO activity.|
|Kit components||• Extraction Buffer I
• Extraction Buffer II
• Assay Buffer
|Features & Benefits||• Determination of MAO activity in a variety of biological samples such as serum, plasma, tissues, cells, bacteria, plants and Fungi.
• Detailed sample preparation and result calculation methods are provided.
• Simple operation steps.
|Usage notes||• 96 Well Clear Flat Bottom UV-Transparent Microplate is needed.
• Do not mix or substitute reagents or materials from other kit lots or vendors.
• The sample preparation must be done on ice to prevent the enzyme inactivation, and the enzyme activity should be determined on the same day. avoid repeated freeze-thaw.
• Ensure all reagents and equipment are at the appropriate temperature before starting the assay.
|Storage instructions||Storage at -20°C and protected from light upon receipt. Kit has a storage time of 12 months form receipt.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||MAO (EC220.127.116.11) mainly exists in various organs of vertebrates, especially secretory glands, brain and liver. It also exists in plants such as invertebrates and legumes. Although the content of MAO is low in vivo, it has important physiological functions and its activity can reflect the degree of liver fibrosis. In addition, the abnormal activity of MAO leads to the disorder of the monoamine neurotransmitter in the cell, which leads to a variety of diseases.|
Fig. CheKine™ Monoamine Oxidase (MAO) Activity Colorimetric Assay Kit
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