|Product name||CheKine™ Lipid Peroxidation (MDA) Assay kit|
|Applications notes||CheKine™ Lipid Peroxidation (MDA) Assay Kit provides a convenient tool for detection of the malondialdehyde (MDA) present in a variety of samples. MDA, together with 4-hydroxynonenal (4-HNE), is a natural bi-product of lipid peroxidation and its quantification is generally used as marker for lipid peroxidation. The MDA in the sample reacts with thiobarbituric acid (TBA) to generate a MDA-TBA adduct，which can be easily quantified colorimetrically at OD 532 nm. Meanwhile, the absorbance at 600 nm was measured , and MDA was calculated by the difference between the absorbance at 532 nm and 600 nm (To avoid the interference of saccharose).|
|Kit components||• Lysis Buffer
• Reaction Mix
• 1 mM Standard
|Features & Benefits||• Determination of MDA in serum, plasma, urine, tissue/cell lysates and other biological fluids.
• A broad range linearity: 1-100 µM/L.
• Fast and convenient, measuring lipid peroxidation in a wide range of samples.
|Usage notes||• The Reaction Mix may form precipitate, you can sonicate it in a 70°C water bath.
• If not assayed immediately, samples can be stored at -80°C.
|Storage instructions||Storage at -20°C immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||Oxidative stress has been implicated in the etiology of many chronic diseases, including cardiovascular disease. Lipid peroxidation is the degradation of lipids that occurs as a result of oxidative damage and is a useful marker for oxidative stress. Polyunsaturated lipids are susceptible to an oxidative attack, typically by reactive oxygen species, resulting in a well-defined chain reaction with the production of end products such as malondialdehyde (MDA). Lipid peroxidation may contribute to the pathology of many diseases including atherosclerosis, diabetes and Alzheimer’s.|
Fig. Typical MDA standard calibration curve. The y-axis is ΔOD and the x-axis is MDA concentration (uM).
Author：Anton Bahtiar, Putri Sagita Utami, Melati Raisa Noor Publication name：Pharmacognosy Journal IF：